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研究生: 鍾佩伶
Pei-Ling Chung
論文名稱: 酸麵糰微生物之篩選及應用田口灰關聯分析法於天然紅藜酵母麵包品質之研究
Microbial screening of sourdough and the application on Baking Qualities of Djulis Sourdough Bread Using Taguchi Gray Relational Analysis
指導教授: 陳和賢
Chen, Ho-Hsien
廖遠東
Liaw, Ean-Tun
學位類別: 博士
Doctor
系所名稱: 農學院 - 食品科學系所
Department of Food Science
畢業學年度: 109
語文別: 中文
論文頁數: 151
中文關鍵詞: 紅藜田口灰關聯法質地剖面分析法感官品評酸麵糰
外文關鍵詞: Djulis, Taguchi grey relational analysis, Texture profile analysis, Sensory attributes, Sourdough
DOI URL: http://doi.org/10.6346/NPUST202100051
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  • Djulis(Chenopodium formosanum),稱為紅色藜麥或台灣紅藜,首先種植於台灣南部和東部的原住民部落,具有很高的營養價值及特色。於本實驗開發出一種新的烘焙產品為djulis酸麵糰麵包,及結合田口(Taguchi)法和灰色理論來優化烘焙產品配方。運用Taguchi法的五個主要的影響因子為紅藜酸麵糰(A)、脫殼熟紅藜(B)、油脂種類(C)、麵包粉(濕麵筋含量為29.0%)和高筋粉(濕麵筋含量為35.5%)的混合麵粉料(D)和蜂蜜(E),其每個因子有四個水準被選擇用於田口實驗設計(L16(4)5)。相關參數為儀器檢測值,分別有5項質地剖面分析值(脆性、彈性、內聚性、黏彈性和咀嚼性)、3項色澤分析值(L*、a*和b*)和6項感官品評評估項目值(外觀、香氣、苦味、酸味、耐嚼性及總體接受度)。運用田口-灰關聯分析法找出最佳烘焙產品配方條件,創新性優化技術的優勢,研究結果生產具有最佳質地、顏色和感官品質的紅藜酵母麵包的最佳配方因子為A3B1C1D2E2,最佳配方天然紅藜酵母麵包的烘焙配方為20%紅藜酸麵糰、0%脫殼蒸熟紅藜、8%無鹽奶油、80%台灣麵粉+ 20%水手牌高筋麵粉和10%蜂蜜,及固定配方有2%鹽、54%水、新鮮酵母3.5%、奶粉4%、雞蛋12%所製作天然紅藜酵母麵包。其新穎的應用方法可作為烘焙業提高產品品質的參考。

    Djulis (Chenopodium formosanum), known as red quinoa or Taiwan djulis, originally cultivated by Taiwanese indigenous people in mountain areas in eastern and southern Taiwan, has a high nutritional value and characteristic properties. In the present study, a new bakery product (djulis sourdough bread) was developed and a combination of the Taguchi method coupled with grey theory was utilized to optimize the baking parameters (product formulation). Five main factors, i.e., djulis sourdough (A), hulled djulis (B), oil type (C), a mixture of bread flour (wet gluten content of 29.0%) and a high-gluten flour (wet gluten content of 35.5%) (D), and honey (E), (each at four levels) were chosen for the Taguchi experiment design (L16 (4)5). Dependent parameters were the data from texture profile analysis (brittleness, springiness, cohesiveness, gumminess, and chewiness), color analysis (L*, a*, and b*), and sensory evaluation (appearance, aroma, bitterness, sourness, chewiness, and overall acceptance) of the final product. Taguchi grey relational analysis successfully determined the optimal conditions based on combined parameters (5 factors), which highlighted the advantages of this innovative optimization technique. The result shows that the optimal formula for producing a djulis sourdough bread with the best texture, color, and sensory qualities was A3B1C1D2E2, i.e., 20% djulis sourdough, 0% addition of hulled djulis, 8% unsalted butter, 80% wheat flour + 20% high-gluten flour, 10% honey, 2% salt, 54% water, 3.5% fresh yeast, 4% milk powder, and 12% egg, respectively. Such a novel application could be a reference for improving the quality of bakery products in the industry.

    目 錄
    摘 要 I
    Abstract III
    謝 誌 V
    目 錄 VII
    圖目錄 XI
    表目錄 XII
    壹、前言 1
    貳、文獻回顧 8
    一、台灣藜 (Chenopodium formosanum Koidz.) 8
    (一) 台灣藜簡介 8
    (二) 地理分析 9
    (三) 植物性狀 9
    (四) 台灣藜一般營養成分 10
    (五) 台灣藜之生理功效 10
    (六) 台灣藜之機能性成分 12
    (七) 台灣藜機能性分析 15
    (八) 台灣藜之抗氧化酵素 15
    二、甜菜色素 (Betalains) 16
    (一) Betalains 之生(物)合成 16
    (二) 甜菜色素抗氧化作用 19
    三、色澤分析 19
    (一) 色澤殘留率 19
    (二) 色澤裂解指數 19
    (三) Hunter L a b 值之測定 20
    四、烘焙產品定義及分類 20
    五、烘焙原料特性之介紹 21
    (一) 麵粉 (Flour) 21
    (二) 油脂 (Lipids) 24
    (三) 水 (Water) 26
    (四) 糖類 (Sugar) 27
    (五) 鹽類 (Salts) 31
    (六) 蛋 (Egg) 33
    (七) 乳品 (Milk) 34
    (八) 酵母菌與乳酸菌 35
    (九) 酸麵糰 (Djulis sourdough) 41
    六、烘焙產品-麵包製作方法 54
    (一) 直接醱酵法 (Straight dough method) 54
    (二) 中種法 (Sponge dough method) 54
    (三) 酸麵糰 (Sourdough) 55
    七、攪拌作用 55
    八、醱酵作用 55
    九、烤焙作用 56
    十、麵包之老化 56
    (一)麵包老化定義 56
    (二)影響麵包老化因素 56
    (三)抑制烘焙產品老化之方法 57
    十一、天然圓頂吐司麵包品判標準 58
    十二、田口品質工程簡介 60
    (一)、田口實驗法 60
    (二)、因子的種類 60
    (三)、直交表 62
    (四)、SN比 67
    十三、灰色系統理論 68
    參、材料與方法 70
    一、實驗架構及流程 70
    二、材料 73
    三、酸麵糰微生物篩選 73
    四、酸麵糰麵包分析方法 74
    (一) 麵糰產氣量 74
    (二) 天然紅藜酵母麵糰體積膨脹速率 74
    (三) 天然紅藜酵母麵包之顏色分析 75
    (四) 天然紅藜酵母麵包之物性分析 75
    (五) 天然圓頂紅藜麵包感官品評 75
    五、烘焙器具及儀器設備 76
    (一) 烘焙器具 76
    (二) Texture profile analysis (TPA) 儀器 76
    (三) 色差分析儀 (colorimeter) - L*、a*、b* 測定 76
    六、烘焙材料 77
    (一) 紅藜 (Djulis) 77
    (二) 紅藜酸麵糰 (Djulis sourdough) 77
    (三) 麵粉 (Flour) 79
    (四) 奶油/油 (Butter/Oil) 79
    (五) 蜂蜜 (Honey) 80
    (六) 蛋 (Egg) 81
    (七) 奶粉 (Milk powder) 81
    (八) 食鹽 (Salt) 81
    (九) 水 (Water) 82
    (十) 酵母 82
    七、烘焙製作配方 83
    八、田口灰關聯分析法 84
    九、質地剖面分析 (Texture profile analysis; TPA) 92
    十、感官品評試驗 (Sensory evaluation test) 93
    十一、統計分析 94
    肆、結果與討論 98
    一、酸麵糰中微生物篩選之研究 98
    (一) 酸麵糰的pH變化情形 99
    (二) 酸麵糰的發酵力變化情形 101
    (三) 酵母菌及乳酸菌的篩選與分離 105
    (四) 討論 108
    二、應用田口-灰關聯分析法於烘焙天然酵母麵包品質之研究 109
    (一) 天然紅藜酵母麵包之感官品評 112
    (二) S/N值計算 116
    (三) 灰關聯度分析 116
    (四) 灰關聯度因子效果分析 124
    (五) 討論 127
    伍、結論 128
    陸、參考文獻 130
    作者簡介 146

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