植物負極性RNA病毒逆向遺傳系統發展之現況與展望

Current Progress and Prospect of the Reverse Genetics of Plant negative-strand RNA Viruses

張賀雄(H.-H. Chang) ； 古新梅(H.-M. Ku) ； 詹富智(F.-J. Jan)

《植物病理學會刊》 18卷4期 (2009/12) Pp. 201-216

https://doi.org/10.6649/PPB.200912_18(4).0002

摘要

逆向遺傳系統(reverse genetics)在分子病毒學上，專指透過cDNA構築表現基因體RNA而產生病毒的研究系統。自1994年第一個負極性RNA病毒(negative-strand RNA virus)從cDNA構築產生後，逆向遺傳系統從而成爲負極性RNA病毒研究上一個普遍、可靠且有力的工具，用來深入研究病毒的生活史、病毒蛋白的功能性角色以及病毒與寄主間的交互作用。然而截至目前爲止，仍未有植物負極性RNA病毒被建立起有效率地、可供分子應次或基因體功能學研究的逆向遺傳系統。在這篇文章中，我們彙整了近年來負極性RNA病毒被建立逆向遺傳系統的相關研究，並針對它們的發展與應用進行討論；動物負極性RNA病毒逆向遺傳系統建立的成就與經驗，將有助於我們發展、建立植物負極性RNA病毒的逆向傳系統，並在進一步的深入研究中做出巨大的貢獻。

關鍵字

逆向遺傳系統 ； 負極性RNA病毒 ； 番茄斑萎病毒屬病毒

並列摘要

The term, reverse genetics, is used in molecular virology to describe the generation of viruses owning its genome derived from cDNA clones. Since the first development of a negative-strand RNA virus entirely from cDNA clones in 1994, the reverse genetics system becomes a popular, reliable and powerful tool for the negative-strand RNA viruses to dissect the virus life cycle, the role of viral proteins and the interaction between viruses and hosts. To date, there is still no efficient routine reverse genetics system for detailed molecular investigation and functional genomics elucidation of any member of the plant negative-strand RNA viruses. In this article, we reviewed the recent studies related to generating reverse genetics systems for negative-strand RNA viruses, and discussed the development and application of the reverse gene tics. The implementation and experience of developing the reverse genetics of the animal negative-strand RNA viruses will help us to develop that of the plant negative-strand RNA viruses and contribute enormously for the profound study.

並列關鍵字

reverse genetics ； negative-strand RNA virus ； Tospovirus