第二代和第三代樹狀高分子PAMAM對小鼠胚胎發育的影響

Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development

夏安良(Sabuna-Alan Charis)

指導教授 ： 詹文雄

中原大學/工學院/生物科技研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400859

摘要

摘要

　　早期的研究指出，樹狀高分子PAMAM可以運送藥物或基因進入哺乳動物組織系統，然而在近幾年的研究下，發現樹狀高分子PAMAM會導致動物組織細胞受損，但樹枝狀高分子PAMAM對胚胎發育的影響則尚無相關研究資料。因此本研究主要為探討第二代和第三代樹枝狀高分子PAMAM對小鼠囊胚期胚胎的影響，分析樹枝狀高分子PAMAM之胚胎的毒性，探討其對胚胎型態、凋亡、增生、體外發育、氧化壓力變化及粒線體膜電位改變之影響。結果顯示出，樹枝狀高分子PAMAM所產生之毒性與表面所帶有之胺基數具正相關性（第二代 < 第三代）；第二代樹枝狀高分子PAMAM與小鼠囊胚共培養下顯示出較低毒性，研究也發現第二代樹枝狀高分子PAMAM並不會改變小鼠囊胚細胞內氧化壓力與造成粒線體膜電位的改變。因此本研究明確指出第二代樹枝狀高分子PAMAM對於小鼠囊胚期胚胎具有相當低的毒性。

關鍵字

樹枝狀高分子PAMAM、小鼠囊胚期胚胎、胚胎毒性、細胞存活率、凋亡

並列摘要

ABSTRACT

PAMAM dendrimer has been recently shown can deliver drug or gene into the targeted mammalian tissues. However, certain studies revealed that PAMAM dendrimers impaired mammalian cells. Moreover, in terms of teratogenicity study little information focused on the cytotoxicological study of PAMAM dendrimers on mammalian embryos. Thus, this study is conducted to examine the influence of generation 2 (G2) and generation 3 (G3) of PAMAM dendrimer on mouse blastocyst. To investigate the influence of PAMAM-induced cytotoxicity, cell morphology, apoptosis, proliferation, in vitro development, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) change were examined. The results indicate that a generation dependent cytotoxicity of the PAMAM dendrimers was found to be correlated well with the number of amine group at surface area (G2

並列關鍵字

embryotoxicity ； cell viability ； mouse blastocysts ； Poly(amidoamine) dendrimers ； apoptosis.

參考文獻

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