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研究生：	謝文昊 Hsieh, Wen-Hao
論文名稱：	GR多肽與其上游C9ORF72基因的動態交互作用 Dynamic Interactions between Poly-GR Peptide and its Upstream C9ORF72 Gene
指導教授：	李以仁 Lee, I-Ren
學位類別：	碩士 Master
系所名稱：	化學系 Department of Chemistry
論文出版年：	2019
畢業學年度：	107
語文別：	中文
論文頁數：	67
中文關鍵詞：	肌萎縮性脊髓側索硬化症、額顳葉失智症、C9ORF72 、六核苷酸重複序列擴張、單分子螢光共振能量轉移、二肽重複蛋白
英文關鍵詞：	ALS, FTD, C9ORF72, hexanucleotide repeat expansions, smFRET, DPR proteins
DOI URL：	http://doi.org/10.6345/NTNU201900426
論文種類：	學術論文
相關次數：	點閱：60 下載：0
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謝誌    i
摘要    ii
Abstract    iii
目錄    iv
圖目錄    vi
表目錄    viii

第一章、緒論    1
1 C9ORF72 (Chromosome 9 open reading frame 72) 與肌萎縮性脊髓側索硬化症及額顳葉失智症關連性    1
2核苷酸序列的重複擴張與衍生疾病    3
2. 1 核苷酸重複序列與其相關疾病介紹    3
2. 2 核甘酸序列擴張機制與二級結構關係    3
3 C9ORF72致病機制    5
3. 1 C9-ALS / FTD致病成因    5
3. 2 C9ORF72產生雙肽蛋白與聚集    6
3. 3蛋白質聚集的形成與神經疾病    8
4 (G4C2)n重複序列與poly—GR交互作用研究    9

第二章、實驗方法與儀器    10
1 實驗儀器與原理    10
1. 1 單分子實驗技術    10
1. 2 單分子螢光共振能量轉移    11
1. 3 全內反射螢光顯微鏡    15
2 實驗器材與樣品置備    17
2. 1 玻片前處理    17
2. 2 DNA的設計    20
2. 3 螢光染料的標記    22
2. 4 DNA黏合反應    23
2. 5 顯像緩衝溶液 (imaging buffer)    24
2. 6 重複二胜肽溶液配置與保存    28
2. 7 實驗流程    28
3 數據處理與分析    31

第三章、實驗結果與討論    36
1 實驗設計    36
2 d(GGGGCC)n 髮夾構型鑑定    37
3重複二胜肽對d(GGGGCC)4 不同二級結構的影響    38
4 (GR)30 與DNA髮夾結構交互作用的專一性探討    39
5 重複二胜肽長度對DNA髮夾結構的影響    40
6 重複二胜肽濃度控制及動力學分析    40
7 再注入實驗    43
8 單分子動態追蹤    44

第四章、結論與未來展望    63

參考文獻    65
                                

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2024/07/31

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