Evaluation of Zinc Inhibition Concentrations on PAH-Degraders by Plate-Dilution Frequency Technique

以平板稀釋數菌法評估鋅對多環芳香烴分解菌之抑制濃度

張時獻(Shih-Hsien Chang) ； 王凱淞(Kai-Sung Wang) ； 戴巧雯(Chiao-Wen Tai) ； 盧至人(Chih-Jen Lu)

《環境保護》 28卷2期 (2005/12) Pp. 147-159

https://doi.org/10.30017/EP.200512.0003

摘要

土壤常受有機物及重金屬污染，重金屬會抑制有機污染物生物分解，因此了解土壤有機分解菌對重金屬之忍受程度，對於土壤生物復育十分重要。本研究使用平板稀釋技術可提供一簡單方法了解不同生長速度之多環芳香烴分解菌對鋅之忍受程度。本研究評估三種稀釋液對鋅抑制濃度值之影響。在無鋅存在時，使用蒸餾水或無機鹽為稀釋液對於菌生長影響並無差異，但以0.7%氯化鈉水溶液為稀釋水時，卻會造成嚴重影響，另外若使用無機鹽稀釋液鋅之抑制濃度遠大於蒸餾水稀釋液。慢速生長之多環芳香烴分解菌對於鋅之忍受程度高於快速生長菌，多環芳香烴分解菌以naphthalene為碳源時，其對鋅之敏感程度高於以phenanthrene為碳源，本結果顯示鋅之生物抑制濃度受到碳源種類之影響。

關鍵字

重金屬 ； Naphthalene ； Phenanthrene ； PAH分解菌

並列摘要

Soils are often co-contaminated with organic and metal pollutants. The presence of heavy metals may inhibit biodegradation of organic contaminants. In this study, plate-dilution frequency technique was used to assess zinc tolerances of subpopulations of PAH degraders with different growth rates. The effects of three common diluents on zinc inhibition concentrations (ICs) were also investigated. In the absence of zinc, there were no significant differences in enumeration between distilled water and minimal medium diluents. In contrast, 0.7% sodium chloride diluent seriously retarded the growth of PAH degraders. Moreover, the ICs obtained by using minimal medium diluent were higher than those obtained by using water. The slow growing PAH degraders were more tolerant to zinc than the fast growing degraders. PAH degraders growing on naphthalene were more sensitive to zinc than those growing on phenanthrene, showing that the ICs of zinc were also influenced by the carbon sources used. This study provides valuable information on success of bioremediation of PAH-contaminated soil in the presence of metals.

並列關鍵字

Metal ； Naphthalene ； Phenanthrene ； PAH degrader