Study of Morphology and Deciduomal Proteins of Hamster Endometrial Cells during in Virtro Decidualization

田鼠子宮內膜細胞體外蛻膜化之形態與蛻膜瘤蛋白之研究

陳碩甫(Shuo-Fu Chen) ； 卓貴美(Guey-Mei Jow) ； 陳同仁(Tong J. Chen)

《The Chinese Journal of Physiology》 33卷2期 (1990/06) Pp. 131-143

摘要

本實驗旨在探討蛻膜瘤細胞的形態特徵是與分化有關的變化抑或只是增生和增大有關的變化，並探討蛻膜瘤相關蛋白在體外蛻膜化過程和體內蛻膜化過程的表現是否一致。體外蛻膜化的方法是取假孕第四日之子宮內膜做分離細胞培養。比較這些細胞與培養的胚胎纖維母體細胞的形態，發現它們十分相似：多爲星形細胞且皆有雙核細胞及細胞內纖維出現。蛻膜瘤相關蛋白的認定是經由比較單側誘發之蛻膜瘤細胞與其對側未誘發之子宮內膜細胞之電泳譜而得。結果發現在細胞質上清液中有四種蛻膜瘤相關蛋白存在。分析這四種蛋白在胚胎纖維母細胞中的表現，發現只有一種是隨著生長而增加；並分析它們在其他組織中的表現，發現它們都不是子宮內膜特有的。分析它們在體外蛻膜化過程中的表現情形，發現與在體內蛻膜化過程中的表現不一致。這些結果顯示：(1)過去被用來認定爲蛻膜瘤細胞的形態特徵，可能有些只是與增生有關的變化而非與分化有關的變化；(2)體外蛻膜化過程與體內蛻膜化過程並不完全相當。

並列摘要

The objective of this study was first, to examine whether the morphological characteristics of deciduomal cells are correlate to differentiation or just to hyperplasia and hypertrophy, and second, to examine whether the deciduoma-associated proteins (DAPs) are expressed consistently during decidualization in vitro and in vivo. In vitro decidualization was implemented by dissociated cell culture of Day-4-pseudopregnant endometrium. The morphology of these cultures was compared with that of undifferentiated cultures of fetal hamster fibroblast. They appeared similar morphology in the stellate shape, the binucleation and the intracellular fibers. The DAPs were identified by comparing the electrophoretic maps of one-day cultures of decidualized and nondecidualized endometrium from unilaterally scratched Day-6-pseudopregnant animals. Analysis of cytosol fraction by SDS-PAGE demonstrated the presence of four DAPs. That only one of them was growth-associated, which was concluded from its increasing expression in fetal fibroblast, and that all of them were not endometrium-specific as evidenced by their presence in other tissues. By examining their expression during in vitro decidualization, we found that timing of the DAPs' expression in vitro was not consistent with it in vivo. These results indicate that some morphological characteristics which were accustomedly used to mark deciduomal cells may relate to hyperplasia and hypertrophy instead of differentiation, and that the in vitro and the in vivo decidualization are not identical.

並列關鍵字

endometrial cells ； decidualization ； cell culture ； morphology ； proteins ； electrophoresis ； hamsters