A Rapid Flow Injection Spectrophotometric Analysis for Tetracycline Chlortetracycline or Oxytetracycline

利用快速分流注射光譜儀分析四環黴素、氯黴素及羥四環黴素

Wirat Ruengsitagoon

《藥物食品分析》 16卷6期 (2008/12) Pp. 16-21

摘要

快速分流注射(FI)光譜儀可偵測四環黴素、氯黴素或羥四環黴素。將150 μL標準品或樣品溶液注入含三價鐵的試劑中（500 μg/mL, 5.0×10^(-3)mol/L硝酸）其最適流速爲4.0 mL/min。三價鐵之四環黴素複合體可在423nm被測得。分流注射系統及實驗條件的最適化依實驗方法而改變。在最適條件下，由曲線圖得到之結果爲1.0-100.0 μg/mL，及其各藥物之偵測極限(s/n=3)爲0.1 μg/mL。TC、CTC、OTC相關係數分別爲0.995、0.994和0.990。此方法可成功運用在測定這些藥物的生產量及配藥上。

關鍵字

分流注射 ； 四環黴素 ； 氯黴素 ； 羥四環黴素

並列摘要

A rapid flow injection (FI) spectrophotometric procedure for the determination of tetracycline (TC), chlortetracycline (CTC) or oxytetracycline (OTC) has been developed. The method is based on the injection of 150 μL standard or sample solution into a reagent stream of iron (Ⅲ) (500 μg/mL in 5.0×10^(-3) mol/L HNO3) with the optimum flow rate of 4.0 mL/min. The brown iron (Ⅲ)-tetracycline complex is monitored at 423 nm. The flow injection system and the experimental conditions were optimized by means of univariate method. Under the optimum conditions, calibration graphs were obtained for 1.0-100.0 μg/mL and the detection limits (s/n=3) were 0.1 μg/mL of each drug. The correlation coefficients were 0.995, 0.994 and 0.990 for tetracycline, chlortetracycline and oxytetracycline, respectively. The method was successfully applied to the determination of these drugs in pharmaceutical formulations with a sample throughput of 70/h.

並列關鍵字

flow injection ； tetracycline ； chlortetracycline ； oxytetracycline