Hepatoprotective Activity of Fresh Polygonum Multiflorum against HEP G2 Hepatocarcinoma Cell Proliferation

生鮮何首烏抑制肝癌Hep G2細胞增生之護肝活性

李保宏(Bao-Hong Lee) ； 黃雅吟(Ya-Yin Huang) ； 吳思敬(She-Ching Wu)

《藥物食品分析》 19卷1期 (2011/03) Pp. 26-32

摘要

生鮮何首烏的根部水萃物(WRP)對兩株肝細胞氧化壓力的調節活性第一次被探討。WRP可提升Clone 9胞內抗氧化酵素活性，包括catalase (CAT)，glutathione-S-transferase (GST), glutathione reductase (GR)及glutathione peroxidase (GPx)，且可增加Clone 9胞內GSH含量，此是因WRP處理24小時可有效提升抗氧化酵素所致。除此之外，WRP處理Hep G2細胞可大量增加活性氧，進而抑制細胞增生。WRP (1000 μg/mL)處理24與48小時，可分別增加78%及77%的凋亡比率。再者，WRP可刺激caspase-8、caspase-9及caspase-3活性，且WRP對Hep G2細胞增生的抑制效應與caspase誘導活性優於其emodin類似物(WRPE)。這些結果顯示何首烏根中所含的植物化學物質可協同的促進肝癌細胞凋亡，而這些萃取物可被使用爲肝臟防護素。

關鍵字

何首烏 ； 抗氧化酶 ； 生長抑制 ； 細胞凋亡 ； 保肝

並列摘要

The modulatory activity of water extracts from fresh roots of Polygonum multiflorum (WRP) on oxidative stress in two liver cell lines was investigated for the first time. WRP increased antioxidant enzyme activities in Clone 9 cells, including catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx). The intracellular glutathione (GSH) level was also increased in the Clone 9 cells owing to antioxidase activity elevating effect by WRP treatment for 24 h. On the other hand, reactive oxygen species (ROS) were generated in abundance upon WRP treatment in Hep G2 cells, resulting in the suppression of cell proliferation. Apoptosis was increased to 78% and 77% after WRP (1000 μg/mL) treatment for 24 and 48 h, respectively. Moreover, WRP stimulated the activation of caspase-8, caspase-9, and ultimately caspase-3. Hep G2 cell growth suppression and caspase induction by WRP were greater than that by emodin-like substances (WRPE), a major active compound. These results indicated that phytochemicals present in extracts of P. multiflorum root act synergistically to promote apoptosis, suggesting the possible use of these extracts for hepatoprotection.

並列關鍵字

Polygonum multiflorum ； antioxidant enzyme ； growth suppression ； apoptosis ； hepatoprotection