Probenazole-Induced Resistance of Lily Leaves against Botrytis elliptica

誘導百合系統性抗灰黴病之研究

路幼妍(Yu-Yen Lu) ； 陳昭瑩(Chao-Ying Chen)

《植物病理學會刊》 7卷3期 (1998/09) Pp. 134-140

https://doi.org/10.6649/PPB.199809_7(3).0003

摘要

本研究探討施用撲殺熱促進百合抗灰黴病的效果。以撲殺熱(4、40及400毫克/公斤土壤)處理百合根圈，可明顯地減少灰黴病菌Botrytis elliptica感染百合葉片所造成的病斑數目；但對病斑大小並無顯著的影響。連續於接種前7、4、1天處理撲殺熱(40毫克/公斤土壤)三次者，較分別於接種前7、4、1天處理一次者更能有效地減少病斑數目。小規模田間試驗顯示，施用撲殺熱(40毫克/公斤土壤)並不影響百合的正常生長，且能有效地降低B. elliptica對百合的感染程度。實驗室試驗結果顯示撲殺熱濃度為2或20μg/ml時不會抑制B. elliptica孢子發芽或菌絲的生長；僅當濃度提高至200μg/ml時，則有些微的抑制作用，顯示撲殺熱可能經由誘導抗病性的作用而達到保護植物的效果。然而，以撲殺熱處理並不會明顯地造成百合葉片內水楊酸含量的增加。

關鍵字

百合 ； 灰黴病 ； 撲殺熱 ； 水楊酸 ； 誘導抗病性

並列摘要

The protection effect of probenazole on lily plants against B o t rytis elliptica was examined in this study. Application of probenazole in the rhizosphere of lily plants at concentrations of 4, 40 or 400 mg per kg of soil at day 7 before inoculation of B. elliptica was able to reduce the number of lesions on plants. However, the treatment did not influence the size of lesions. Furthermore, applying this chemical three times at the concentration of 40 mg per kg of soil at days 7, 4, and 1 pre-inoculation more obviously reduced the number of lesions than treating one time with probenazole. Probenazole, at the concentration of 40 mg per kg of soil, protected lily plants from the infection of B. elliptica without retarding the growth of plants. In vitro assays revealed that probenazole at the concentration of 2 or 20 μg/ml did not inhibit spore germination in distilled water and mycelial growth of B. elliptica on V-8 agar. Spore germination and mycelial growth became inhibited only if the concentration of probenazole was raised to 200 μg/ml. The protection of lily plants by probenazole may be attributed to the resistance of host plants induced by the chemical. However, the level of salicylic acid in the lily leaves did not significantly increase after the treatment of probenazole.

並列關鍵字

lily ； Botrytis leaf blight ； gray mold ； oryzemate ； salicylic acid ； induced disease resistance