鰻魚黏液之生化與生理活性探討

Study on the Biochemistry and Physiology Activities of Japanese Eel (Anguilla japonica) Mucus

李雅婷(Ya-Ting Lee) ； 葉彥宏(Yen-Hung Yeh) ； 黃議輝(Yi-Hui Huang) ； 黃豋福(Deng-Fwu Hwang)

《建國科大學報》 25卷3期 (2006/04) Pp. 95-110

https://doi.org/10.6995/JCTU.200604.0095

摘要

台灣的鰻魚養殖以日本鰻(Anguilla japonica)為主，其在食品加工上，鰻魚算是一種具高經濟價值的魚類。唯加工時所產生之黏液，仍被視為降低生產製成率的廢棄物。
本研究首先瞭解鰻魚黏液之一般組成與其所具有的生理活性特性，由研究中指出其一般組成90%以上為水分及蛋白質。此外，以緩衝溶液抽取黏液中的蛋白質，作生理活性分析，發現其中具有溶血物質、毒性物質及脂解酵素(lipase)、鹼性磷酸酵素(alkaline phosphatase，ALP)和天門冬胺酸轉移酵素(aspartate transferase，AST)等酵素活性物質存在。其中溶血活性物質，會隨時間及溫度有活性上的變化。在電泳圖中，也顯示其中主要具有92、48、32、21KDa與其他多種蛋白質的存在。而在抗菌分析中，並無發現對腸炎弧菌、大腸桿菌、金黃色葡萄球菌有明顯抗菌的效果。其次以酵素水解蛋白質，測知其水解液中游離胺基酸組成主要為threonine、leucine、isoleucine、serine和glycine等。

關鍵字

鰻魚 ； 鳗魚黏液 ； 生理活性

並列摘要

Japanese eel (Anguilla Japonica) is one of the majority cultured eel in Taiwan. On the food processing, eel contains higher economical value than other fish. The eel mucus which still is one kind of waste when eel is processed.
In this study, the composition and physiology of eel mucus have been investigated fistly. It was found that water and proteins are major components more than 90 percents in the eel mucus. Besides, the mucus are extracted with different buffer solutions for biochemistry analysis. The extracts were found to have hemolytic substances, toxin, lipase, alkaline phosphatase (ALP) and aspartate transferase (AST), etc. Among them, the hemolytic substance would change with storage time and temperature. After analysis of electrophoresis, it indicated to contain 92 KDa, 48 KDa, 32 KDa, 21 KDa and other proteins. After antibacterial test, the eel mucus did not have definite antibacterial activity for Vibrio parahaemolyticus, Escherichia coli and Staphylococcus aureus in the extracts. After enzyme digestion, the major free amino acid of digesting solution included threonine, leucine, isoleucine, serine and glycine.

並列關鍵字

eel ； eel mucus ； physiology activity