桂竹葉中黃酮成份之抗氧化活性探討

The Antioxidant Activities of Flavonoids in Phyllostachys Makinoi Hayata

劉柏村(Po-Tsun Liu) ； 黃俊薰(Chun-Hsun Huang) ； 何睿芬(Ruei-Fen He) ； 黃文盈(Wen-Ying Huang)

《長庚科技學刊》 19期 (2013/12) Pp. 31-34

https://doi.org/10.6192/CGUST.2013.12.19.4

摘要

本研究以高效液相層析儀分析桂竹之黃酮成分，利用流動相A（acetonitrile/methanol = 2:1）與流動相B（H2O），以流速0.8 mL/min沖提50分鐘，在254 nm下可分析2個成分：vitexin與isovitexin。再以清除DPPH自由基方法測定桂竹之抗氧化力，實驗結果顯示，清除DPPH自由基能力IC50為0.540 ± 0.027 mg/mL，可知桂竹具有抗氧化力。

關鍵字

高效液相層析 ； 黃酮 ； 桂竹 ； 抗氧化力

並列摘要

In this study, high performance liquid chromatography was used to analysis the flavonoids of Phyllostachys makinoi Hayata. Eluting with mobile phase A (acetonitrile / methanol = 2:1) and mobile phase B (H2O) at a flow rate of 0.8 mL / min for 50 minutes and 254 nm, the following four components could be analyzed: vitexin and isovitexin. Furthermore, the DPPH radical scavenging activity was examination to determine the antioxidant activity of P. makinoi Hayata. The result showed the P. makinoi Hayata had antioxidant activity and IC50 of DPPH scavenging capacity was 0.540 ± 0.027 mg /mL.

並列關鍵字

high performance liquid chromatography ； flavonoid ； Phyllostachys makinoi Hayata ； antioxidant activity

參考文獻

Wu, J. H., Chung, M. J., Chang, S. T., Evaluation of the effectiveness of alcoholborne reagents on the green colour protection of makino bamboo (phyllostachys makinoi ), Polymer Degradation and Stability 83 (2004) 473-479.

Hunter, I. R., Bamboo resources, uses and trade: the future, Journal of Bamboo and Rattan, 2 (4) (2003) 319.

Chung, M. J., Wu, J. H., Chang, S. T, Green colour protection of makino bamboo (Phyllostachys makinoi ) treated with ammoniacal copper quaternary and copper azole preservatives, Polymer Degradation and Stability, 90 (2005) 167-172.

Yen, T. M., Ji, Y. J., Lee, J. S., Estimating biomass production and carbon storage for a fast-growing makino bamboo (phyllostachys makinoi ) plant based on the diameter distribution model, Forest Ecology and Management, 260 (2010) 339-344.

Gill, S. S., Tuteja, N., Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants, Plant Physiol Biochem., 48 (2010) 909-930.