Disinfected mycorrhizal fungi spores of Glomus fascuculatum were treated with different kinds and concentrations of flavonoids in order to observe the germination rate and hyphal length of the spores. The results showed that spores treated with 5ppm quercetin had the highest germination rate (70%), and the spores treated with 1 ppm (33 μM) and 5 ppm (165 μM) quercetin had longer hyphal length compared with 0 ppm, 10 ppm, and 20 ppm treatments. Further experiment revealed that quercetin 4-6 ppm (132-198 μM) was the most appropriate concentration for improving spore germination, while quercetion 3-6 ppm (99-198 μM) the most adequate for hyphal growth.