Micellar liquid chromatography (MLC) is a branch of high-performance liquid chromatography (HPLC), and it can be used for the simultaneous determination of most drug materials in pharmaceutical preparations, or in biological samples. In this review, the retention behaviour of solutes of different drugs (ionic or neutral exhibiting a wide range of polarities), and the conditions (with ionic and non-ionic surfactants, add salt and organic solvent, and varying pH) are discussed.