含光敏感分子之奈米電紡絲纖維應用於癌症之熱治療

Electrospun Nanofibers Containing photosensitive polymer for hyperthermia in Cancer Therapy

林韋廷(Wei-Ting Lin)

指導教授 ： 蔡偉博

國立臺灣大學/工學院/化學工程學研究所/碩士(2014年)

https://doi.org/10.6342/NTU.2014.01164

摘要

溫熱療法，將癌細胞組織加熱到 41~45 oC 可以明顯增加使用放射或是化學
性治療法的效果。本論文目的是為了發展出含有光敏感分子之電紡絲應用於溫熱
至療法來治療表面癌症腫瘤，例如皮膚癌。聚左乳酸與銅葉綠素鈉所噴成的電紡
絲，可以經由照射 532 nm 的綠光雷射來加熱，達到殺死細胞的目的。
在第二部分我們將苯乙烯與銅葉綠素鈉聚合以後，得到接枝有銅葉綠素鈉單
體的聚苯乙烯，並且將此產物噴成電紡絲後來應用，之後經由 MTT assay、染
trypan blue、live/dead 三種實驗來觀察綠光雷射強度與照射時間對細胞的影響，最後達到可以控制照射綠光雷射的強度與照射時間，來達到殺死癌細胞的目的，並且不會對周遭正常細胞造成明顯損害，我們可以將此結果應用於溫熱治療法來治療大範圍表面癌症腫瘤。

關鍵字

電訪絲 ； 光熱療法 ； 綠光雷射

並列摘要

Hyperthermia, the heating of cancerous tissues to between 41 and 45 oC, has
been shown to improve the efficacy of cancer therapy when used in conjunction with
irradiation and/or chemotherapy. The objective of this study was to develop an
incorporation of electrospun sheet with photosensitizer for photodynamic therapy in
surface cancer tumor, such as skin cancer. The blend of poly-L-lactic acid (PLLA)
and chlorophyllin sodium copper (CSC) was electrospinning a sheet was developed.
The electrospun sheets could be remotely heated upon exposure to an external wave
length 532 nm green laser to kill cells.
In the second part of the study, for the purpose to produce high percentage
chlorophyllin sodium copper contain polystyrene , we used chlorophyllin sodium
copper and styrene with ATRP, and electrospinning to sheet for the hyperthermia
purpose. Then we used MTT assay、trypan blue stain and live/dead stain to observe
the effect of green laser intensity and irradiation time to the cell viability. Finally we
can achieve the purpose of killing cancer cell by control the green laser intensity and
irradiation time without hurting the normal cell around. We can use this result on the
hyperthermia of in surface cancer tumor, such as skin cancer.

並列關鍵字

electropsun ； photothermal therapies ； green laser

參考文獻

effect on antioxidant defence parameters of Wistar rats. Journal of the Science of

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[2] Lepock JR. Cellular effects of hyperthermia: relevance to the minimum dose for

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