多功能上轉換奈米粒子之生物影像與生物醫學應用

Multifunctional Upconversion Nanoparticles for Bioimaging and Biomedical Applications

詹明賢(Ming-Hsien Chan)

指導教授 ： 劉如熹

國立臺灣大學/理學院/化學研究所/博士(2018年)

https://doi.org/10.6342/NTU201800986

摘要

根據衛生福利統計部2016年所提供之死亡統計信息，惡性腫瘤目前是全球人口死亡之主要原因，其次為心血管疾病與肺炎。最近，奈米技術已被應用於醫學檢測、疾病篩檢與環境污染物分析。奈米技術之中，光致發光成像於生物應用方法居重要地位。與傳統檢測方法相比，光致觸發方法之侵入性小且副作用少。本論文旨於發展近紅外光激發下表現出上轉換之奈米粒子其材料設計、性質調整與應用之最新進展。近紅外光具備最佳化之生物光學窗口，並因其可深入穿透組織而有效地提供難以捉摸之生物信息。該特性被用於降低背景噪音，因該波長波段不會被生物分子如血紅蛋白所吸收而遮蔽。近紅外光激發之上轉換奈米粒子(UCNPs)顯示出用於生物傳感、生物成像、治療與三維顯示之優越潛力。本論文中，上轉換奈米粒子將與其他奈米材料(如氮化碳量子點、奈米氣泡與金納米棒)共軛結合，以優化其光照應用條件。此些奈米平台可被應用於生物成像及體外與體內分析治療。於短期與長期之培養過程中，奈米複合材料揭示其構成具備高度物理、化學與生物穩定性。此些上轉換奈米複合物所呈現之奈米載體啟發該研究於癌症光學治療中之深度評估。因此，可以透過將上轉換奈米粒子與光療劑結合以開發創新型奈米平台。期望該奈米複合技術可結合各種材料所具備之獨特優勢。

關鍵字

上轉換奈米粒子 ； 奈米複合材料 ； 生物成像 ； 光學治療 ； 癌症治療

並列摘要

According to the information provided by the Ministry of Health and Welfare in 2016, malignant tumors are currently the leading cause of death worldwide, followed by cardiovascular diseases and pneumonia. Recently, nanotechnology has been applied in medical testing, disease screening, and environmental pollutant analyzing. In nanotechnology, photoluminescence imaging is one of the most important biological application methods. The light-triggered approach is less invasive and induces fewer side effects than traditional detection methods. This thesis refers to, the recent advances in design, property tuning, and applications of nanoparticles that exhibit upconversion under near-infrared light excitation. The near-infrared light achieves the optimal biological window and effectively provides elusive information because it can deeply penetrate the tissue. This characteristic reduces the background noise because the wavelength is not absorbed by biomolecules for example hemoglobin. The near-infrared excited upconversion nanoparticles (UCNPs) exhibit superior potential for use in biosensing, bioimaging, therapy, and three-dimensional display. In this thesis, the UCNPs were conjugated with other nanomaterials, such as carbon nitride dots, nanobubbles, and gold nanorods, to optimize the light application conditions. These nanoplatforms can be applied in bioimaging and therapies for in vitro and in vivo analysis. During both short and long incubation, the nanocomposites reveal physical, chemical, and biological stability. These nanovehicles have inspired the approach used in this investigation for assessing the expression of upconversion nanocomposites in cancer phototherapy. Thus, the innovative nanoplatforms can be developed by incorporating UCNPs with phototherapy agents. This composite technique is expected to combine the individual advantages of each material.

並列關鍵字

upconversion nanoparticles ； nanocomposite ； bioimaging ； phototherapy ； cancer therapy

參考文獻

1.7 References (Chapter 1)

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