蝴蝶蘭癒合組織再生植株與轉殖體系之建立

Plant Regeneration from Callus Culture and Genetic Transformation of Phalaenopsis

徐善德(Shan-Te Hsu)

指導教授 ： 黃鵬林

國立臺灣大學/生物資源暨農學院/園藝學研究所/博士(2007年)

https://doi.org/10.6342/NTU.2007.10264

摘要

蝴蝶蘭是蘭科植物中最重要的經濟栽培作物，可供商業切花和盆花的生產，行政院農業委員會業已將其選定為台灣四大外銷旗艦農產品之一。在台灣，無論是蝴蝶蘭生產或品種改良等研發，應具有可觀的預期經濟利益。然而，蘭花通常擁有較長之幼年期與生殖周期，此特性某種程度局限了傳統雜交育種的改良，現代遺傳工程則是一條可行的品種創育途徑。然而，體細胞變異、試管內選拔和遺傳工程等作物遺傳改良之技術，皆需要有高效率且穩定地經由癒合組織再生植株的體系為基礎。本論文研究之目的擬建立蝴蝶蘭經由癒合組織再生植株的高效率體系，並架構蝴蝶蘭基因轉殖代工平台，期能整合國內蝴蝶蘭分子育種體系，以因應未來蝴蝶蘭品種多樣化之育種需求，期能奠定我國蝴蝶蘭外銷產業堅實的基礎。

關鍵字

蝴蝶蘭類 ； 癒合組織 ； 擬原球莖體 ； 植株再生 ； 植株基因轉殖 ； 報導基因 ； 綠色螢光蛋白

並列摘要

Phalaenopsis orchid is one of the most important orchids grown for commercial production of cut flowers and potted plants. It has been chosen by the Council of Agriculture, Executive Yuan, as one of the four flagship speciality products for export. There is substantial interest in the production and improvement of these commercially valuable plants in Taiwan. However, orchids usually have long juvenile periods and reproductive cycles, which restrict genetic improvement using traditional sexual hybridization. Modern genetic engineering techniques provide an effective alternative. A callus-mediated plant regeneration protocol is a critical requirement as it allows exploitation of in vitro selection, somaclonal variation and engineering techniques aimed at genetic improvement of plants. Although several micropropagation methods have been developed for phalaenopsis using shoot tips, leaf segments, root tips and stalk cuttings, establishment of a proliferating callus culture and regeneration from callus is limited to phalaenopsis. The objectives of the project are to develop an efficient plant regeneration system from callus and to establish a platform of gene transformation for phalaenopsis breeding purpose, hoping to lay a solid foundation for export-oriented businesses.

並列關鍵字

phalaenopsis ； callus ； protocorm-like body ； plant regeneration ； plant transformation ； reporter gene ； green fluorescent protein

參考文獻

徐善德、廖玉琬、黃鵬林. 2007. 應用綠色螢光蛋白報導基因探討蝴蝶蘭癒合組織基因槍轉殖暫時性表達之最佳物理參數. 臺灣園藝 53: 229-236.

許家嘉、陳福旗. 2003. 以蝴蠂蘭白化葉片誘導擬原球體與植株再生. 中國園藝 36: 335-342.

廖玉琬、徐善德、黃鵬林. 1999. 香蕉轉殖細胞葡萄糖苷酸酶活性組織化學染色之改進. 中國園藝 45: 239-244.

Rueb, S., and L. A. M. Hensgens. 1989. Improved histochemical staining for β-D-glucuronidase activity in monocotyledonous plants. Rice Genet. Newsl. 6: 168-169.

謝永祥、黃鵬林. 1995. 應用花粉管法於蝴蝶蘭基因轉殖之研究. 中國園藝 41: 309-324.