犬乳腺腫瘤Ki-67表現與其他新興蛋白質表現之相關性與預後之關係

犬乳腺腫瘤（Canine mammary gland tumors, cMGTs）是雌犬最常發生之腫瘤，其惡性機率從26 %至73 %；目前年齡及卵巢荷爾蒙被認為扮演重要的角色。犬乳腺腫瘤大多延誤治療時機；早期發現且接受正確治療，可避免腫瘤之擴散或轉移。在腫瘤發展進程中，有許多的生物因子可成為惡性腫瘤的生物標記 (Biomarker)，因此，針對可能具有潛在的相關生物標記進行研究，以探討是否可以成為生物標記，有其必要性。本實驗針對176例犬乳腺腫瘤病例作分析，依照WHO犬乳腺腫瘤分類系統與臨床系統分級，配合P63、KLF2、KLF4、KLF5及KLF6等新興蛋白質的表現，結果與Ki-67蛋白質的表現、組織病理學分級及臨床上預後做比較。初步研究結果發現組織病理學下分類與預後有顯著差異 (P < 0.0001) ，說明正確分類可以達到預測預後之效果。但有時亦混淆的情況下，需要輔助性診斷工具，較常使用的為評估細胞增生因子的Ki-67可以成為區分乳腺腫瘤之良惡性輔助工具；與預後比較，認為大於40 %的Ki-67表現量可以預測其預後較差。Ki-67亦與臨床上分級、組織病理學分類、腫瘤分化與預後皆有顯著相關性。KLF4、KLF5及KLF6在細胞核的表現與惡性腫瘤有相關性，推論這些新興抗體在腫瘤演進過程中，扮演重要角色。將Ki-67與KLF2、KLF4、KLF5及KLF6在細胞核表現情形比較結果皆有非常顯著之相關性 (P < 0.0001)。

關鍵字

犬乳腺腫瘤；組織學分類；免疫化學染色； Ki-67 ； KLF2 ； KLF4 ； KLF5 ； KLF6

並列摘要

Canine mammary gland tumors (cMGTs) are the most common neoplasms in intact female dogs and the malignant rate ranges from 41 to 53 %. The most important factors related to cMGTs are age and ovarian hormones. Early detection with subsequent therapies are essential to prevent early local recurrence or distant dissemination. However, these prognostic factors may be not always predictable if a tumor will metastasize or not. The Ki-67, a nuclear antigen, is one of the most commonly used markers to evaluate tumor proliferative activity. In this study, the WHO classification and staging system were used to classify the cMGTs. In addition, the emerging proteins including p63, KLF2, KLF4, KLF5, and KLF6 were employed to compare with histological grading, Ki-67 expression and clinical outcomes. Current results showed that the histological grading and Ki-67 expression were highly significant associated with clinical outcomes. And the increased 40 % expression of Ki-67 had shorter survival time of patients. Moreover, we suggested that Ki-67 could be used to identify the malignancy and histological grading. Otherwise, the nuclear expression of KLF2, KLF4, KLF5, and KLF6 expression was significantly associated with the malignancy of the cMGTs (P < 0.0001). These emerging proteins, considered the important role in the cancer regulation, also showed significant associated with clinical outcomes through comparison with the expression of Ki-67.

並列關鍵字

Canine mammary gland tumors (cMGTs) ； Histological grading ； Immunohistochemistry ； Ki-67 ； P63 ； Kruppel-like factors (KLFs) ； KLF2 ； KLF4 ； KLF5 ； KLF6

參考文獻

1.Abd El‐Rehim DM, Pinder SE, Paish CE, Bell J, Blamey R, Robertson JFR, Nicholson RI, Ellis IO. Expression of luminal and basal cytokeratins in human breast carcinoma. J Pathol 203: 661-671, 2004.

2.Alenza M, Pena L, Castillo N, Nieto A. Factors influencing the incidence and prognosis of canine mammary tumours. J Small Anim Pract 41: 287-291, 2000.

3.Alenza P, Rutteman G, Kuipers-Dijkshoorn N, Pena L, Montoya A, Misdorp W, Cornelisse C. flow cytometry of canine mammary tumours: the relationship of ploidy and S-phase fraction to clinical and histological features. Res Vet Sci 58: 238-243, 1995.

4.Au N, Gown A, Cheang M, Huntsman D, Yorida E, Elliott W, Flint J, English J, Gilks C, Grimes H. P63 expression in lung carcinoma: a tissue microarray study of 408 cases. Appl Immunohisto M M 12: 240, 2004.

5.Bacha Jr WJ, Bacha LM. Color atlas of veterinary histology. Wiley-Blackwell, 2011.

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犬乳腺腫瘤Ki-67表現與其他新興蛋白質表現之相關性與預後之關係

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