佛手柑精油對肺癌細胞之潛在抗癌活性研究

The Potential Anti-cancer Activity of Bergamia Essential Oil to Lung Cancer Cells

賴偲庭(Sih-Ting Lai 賴偲庭)

指導教授 ： 蕭明文

中山醫學大學/健康管理學院/應用化學系碩士班/碩士(2014年)

摘要

佛手柑精油(Bergamia Essential Oils)具有萜類、醇類成分，如沉香醇可能具有化學預防特性，在本研究中，利用A549肺腺癌細胞株觀察佛手柑精油是否有抗腫瘤的潛力。首先，以DAPI染色、Annexin V/PI 雙染法用流式細胞儀偵測等，發現佛手柑精油明顯抑制A549細胞生長並且誘導細胞凋亡。並且以西方墨點法發現經佛手柑精油處理過後會去抑制NF-κB的磷酸化表現。綜合以上結果，佛手柑精油會去誘發細胞凋亡，可視為在癌症治療及預防中有效的醫療資源。

關鍵字

佛手柑精油 ； 肺癌 ； 細胞週期 ； 細胞凋亡

並列摘要

Bergamot essential oil contains terpenes and the compositions of alcohol such as linalool which may possess chemoprvention properties. In this study, I adopt the methods as DAPI staining, Annexin V /P double staining and western blot, etc. The results show Bergamot essential oil will induce apoptosis, that can be considered effective in cancer treatment and prevention of medical resources.

並列關鍵字

Bergamia Essential Oils ； lung cancer ； cell cycle ； Apoptosis

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