胜肽衍生物的合成及純度鑑定之方法

Synthesis of peptide derivatives and determination the purity of peptide

陸怡方(Yi-Fang Lu)

指導教授 ： 高佳麟

高雄醫學大學/生命科學院/醫藥暨應用化學系碩士班/碩士(2019年)

摘要

本篇利用固相胜肽合成法合成胜肽序列，將該序列特定位置換成不同胺基酸，合成一系列的胜肽序列，為鑑定胜肽純度，使用高效能液相層析儀(HPLC)檢測，然而，HPLC檢測其純度的方式一般是利用峰面積去進行計算。但當使用不同層析條件時，利用峰面積去計算所得純度不一定相同，容易造成分析化合物時錯誤的判斷。因此，本篇研究使用HPLC連接紫外光偵測器的光電二極體陣列檢測器，就可以使用3D全光譜判斷波峰的純度以及層析條件的好壞，如果波峰不含其他雜質，除了濃度差異之外，該化合物的全光譜會是相同；當發現化合物內含其他雜質，在不同波長時波峰會不相同，因此本方法可以更精準地分析樣品，提供化合物純度可靠的結果。

關鍵字

胜肽純度 ； 光電二極體陣列檢測器

並列摘要

To study a biological active peptide which was obtained from an infectious animal, a series of analogous were prepared for biological assay.
Therefore, the purity is important for the following works.
With a conventional single wavelength spectra, purity of analyte can be only judged from the peak area of chromatographic. However, different elution conditions cause various different results. Therefore, purity based on the peak area of each signal is an unreliable and insensitive method.
A photodiode array detector can provide additional information such as absorbance, wavelength, and retention time. The peak is pure, apart from concentration differences, the full spectrum is similar to each other. When significant deviations are encountered, this can be seen as an indication of impurity. Thus, this method can analyze purity of compound cross-validated with MS data and NMR data give reliable result of purity of each product.

並列關鍵字

peptide ； purity ； photodiode array detector

參考文獻

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