大黃素對於斑馬魚血液發育之影響

大黃素 (1,3,8-Tri-hydroxy-6-methyl-anthra-quinone) 是大黃中的成分，是一個 p651ck 酪氨酸激酶的抑制子。早期的研究發現大黃素有很多功能，例如抗腫瘤、抗發炎、抑制細胞週期、促進細胞凋亡等等，最近的研究指出大黃素也會影響血管新生及纖維化，但是目前還沒有大黃素和血球方面相關之研究。本研究利用斑馬魚動物模式去探討大黃素是否對於斑馬魚胚胎有所影響。首先我們將斑馬魚胚胎和不同濃度之大黃素共培養，發現在高於 2μM 的大黃素共培養下，斑馬魚胚胎之外觀發育就會改變。雖然有研究顯示大黃素會抑制血管新生的作用，但在本研究中並無發現大黃素對於斑馬魚胚胎之血管發育有任何影響。在本研究中發現，大黃素會影響斑馬魚的血球，與大黃素共培養兩天的組別會產生血球減少甚至是幾乎無血球的現象，我們認為也許大黃素會影響到血球之發育。之後我們利用全複式原位雜交法去分析大黃素是否會專一的影響血球的發育，我們發現大黃素對於 gata1 、 ndrg1 、 mpo 、pu.1 等基因的表現皆無影響，所以我們推測大黃素可能不會影響血球細胞的合成，有可能會影響血球細胞的存活率。

關鍵字

血球細胞；胚胎發育；大黃素；斑馬魚胚胎

並列摘要

Emodin (1,3,8-Tri-hydroxy-6-methyl-anthra-quinone) is a tyrosine kinase p65lck inhibitor which isolated from Rhei Rhizoma. Previous studies have documented many effects of emodin, including anti-tumor, anti-inflammatory, cell cycle inhibition, apoptosis, and reactive oxygen species (ROS) generation. Recent researches show that emdoin also affects on neovascularization and fibrosis, but there is no study showed the effects on blood cells. In this investigation, we used zebrafish model to determine whether there are any injury effects by emodin. Zebrafish embryos that were treated with different concentrations of emodin have found morphological differences. We investigated embryos which treated with over 2μM have morphological changes. Although, emodin suppress angiogenesis, but in our study there have no effects on vascular tube formation were found in zebrafish model. We found that emodin had hazardous effects on zebrafish’s blood cell. There is less or no blood was found in emodin-treated embryos for two days, so we thought maybe emodin affects on mesoderm. And then, we used whole-mount in situ hybridization to determine whether emodin has specific effects on blood cells that development from mesoderm. So far we investigated emodin would not affect expression of gata1, ndrg1, mpo and pu.1. Emodin may not suppress synthesis of blood cells but probably affects on blood cell viability.

並列關鍵字

zebrafish embryos ； blood cells ； embryonic development ； emodin

參考文獻

Bertrand, J.Y., Kim, A.D., Violette, E.P., Stachura, D.L., Cisson, J.L., and Traver, D. (2007). Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo. Development 134, 4147-4156.

Brunstrom, B., and Lund, J. (1988). Differences between chick and turkey embryos in sensitivity to 3,3',4,4'-tetrachloro-biphenyl and in concentration/affinity of the hepatic receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin. Comp Biochem Physiol C 91, 507-512.

Carney, S.A., Prasch, A.L., Heideman, W., and Peterson, R.E. (2006). Understanding dioxin developmental toxicity using the zebrafish model. Birth Defects Res A Clin Mol Teratol 76, 7-18.

Carradice, D., and Lieschke, G.J. (2008). Zebrafish in hematology: sushi or science? Blood 111, 3331-3342.

Chan, T.C., Chang, C.J., Koonchanok, N.M., and Geahlen, R.L. (1993). Selective inhibition of the growth of ras-transformed human bronchial epithelial cells by emodin, a protein-tyrosine kinase inhibitor. Biochem Biophys Res Commun 193, 1152-1158.

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大黃素對於斑馬魚血液發育之影響

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