聚苯乙烯盤披覆苯乙烯與甲基丙烯酸聚乙二醇酯雙嵌段與隨機共聚合物後之抗生物沾黏特性

Anti-biofouling Properties of Polystyrene Plate Coated with Diblock or Random Copolymer Made of Styrene and PEGMA

張宏瑋(Hung-Wei Chang)

中原大學/工學院/生物醫學工程研究所/碩士(2011年)

https://doi.org/10.6840/CYCU.2011.00095

摘要

本研究以苯乙烯(styrene)與甲基丙烯酸聚乙二醇酯(Poly (ethylene glycol) methyl ether methacrylate、PEGMA)聚合成隨機共聚合(random copolymer、PS-r-PEGMA)或雙嵌段共聚合物(diblock copolymer、PS-b-PEGMA)，並將高分子以物理吸附方式批覆於聚苯乙烯培養盤中。所設計之共聚合物結構中，以聚苯乙烯片段提供疏水性作用力吸附於聚苯乙烯培養盤中，而以PEG片段來提供抵抗生物在盤面的沾黏。
本篇主要目的是研究蛋白質、細菌與動物細胞(纖維母細胞、角質細胞)在披覆高分子的培養盤中貼附的情形。我們以原子轉移自由基聚合法(ATRP)聚合共聚合物，在共聚合物的鑑定方面，本研究使用核磁共振(NMR)來分析高分子之化學組成，並以膠體滲透層析儀(GPC)來量測共聚合物之分子量。在高分子批覆於聚苯乙烯盤之表面分析方面，使用X射線光電子能譜(XPS)分析共聚合物之相對批覆量與接觸角量測儀(contact angle)探討材料表面之親疏水性質變化。
結果發現雙嵌段共聚合物具有良好的抗細菌沾黏的能力，適合用於塑膠醫療管線抗菌批覆，特別是當苯乙烯與PEGMA比例為2:1時的效果特別好；而在隨機共聚合物披覆的表面，抑制血漿纖維原蛋白吸附的效果並不佳，因此在使用比例為2:1隨機共聚合物披覆的表面，纖維母細胞在加入10% 胎牛血清的培養情況下，仍舊能夠貼附成長，但若使用無血清培養基，則與角質細胞相同，無法貼附並生長。由此可見，血漿中纖維原蛋白在培養表面的吸附，可能有助於纖維母細胞的貼附。

關鍵字

原子轉移自由基聚合法 ； 抗生物沾黏

並列摘要

In this study, styrene and PEGMA[poly (ethylene glycol) methyl ether methacrylate] were polymerized into random copolymer or diblock copolymer. These polymers were coated by physical adsorption on pure polystyrene plate and the anti-bioadhesion properties were under investigation. The hydrophobic segments of polymers were designed to attach to polystyrene plate by hydrophobic interaction. The PEG moiety was add to reduce polystyrene plate bio-adhesion.
The goal of the work was to study the adhesion of plasma proteins, bacteria, and animal cells onto the polymer coated surfaces. Diblock and random copolymers were synthesized via atom transfer radical polymerization (ATRP) method. The copolymer was identified by 1H nuclear magnetic resonance (NMR) to analysis the chemical composition of copolymers, and gel permeation chromatography (GPC) was used to measure the molecular weight of copolymers. The surface analysis of copolymers coated on polystyrene plate was used X-ray photoelectron spectroscopy (XPS) to analyze the relative quantities of copolymers and investigate the change of hydrophobicity and hydrophilicity on the polymers surface.
It was found that the diblock copolymers had superior antibacterial ability than the random copolymers, especially at the styrene/PEGMA ratio around 2/1. In addition, block copolymers also had higher resistance to plasma proteins than random copolymers. It was found that fibroblasts could adhere and then proliferate on surface coated with the random copolymer of 2/1 styrene to PEGMA ratio while 10% FBS was added in the culture medium. However, fibroblasts do not adhere to the same surface when cultivated in serum free medium. It indicated that the adsorption of plasma proteins facilitated the attachment and subsequent proliferation of fibroblasts.

並列關鍵字

Atom transfer radical polymerization ； anti-bioadhesion

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