天然與改質型黏土對螢光蛋白之吸附行為的研究

Study on the adsorption behavior of fluorescence protein in the pure and modified clays

曾云珍(Yun-Zhen Tseng)

指導教授 ： 蔡宗燕

中原大學/理學院/化學研究所/碩士(2006年)

https://doi.org/10.6840/cycu200600622

摘要

於本研究中運用黏土的矽酸鹽層之層間離子特性，以及黏土具高表面積之特性，使用商業化生產之改質劑: C-50 (Cocamidopropyl -Hydroxysultaine ) ，探討黏土之改質反應條件對黏土插層效果之影響及對於螢光蛋白吸附行為之變異性進行研究。研究結果顯示將黏土控制在pH=7~8 的環境下，進行有機化改質，而且用以稀釋螢光蛋白或膨潤黏土的水均是RO water (Reverse Osmosis water)比DI water (Deionized water)為佳，將其以 RO water 稀釋之螢光蛋白溶液加入有機化改質黏土時，從PL (Photoluminescence)實驗結果可以發現螢光蛋白極容易吸附/插層於有機化改質黏土上面，當以0.2g 螢光蛋白加入 8 ml之 RO water 稀釋的螢光蛋白溶液濃度為0.1083mg/ml，將其添加於有機化改質黏土中，獲得一螢光蛋白濃度為0.3448 mg/ml，兩者濃度相比，被吸附/插層於有機化改質黏土之螢光蛋白濃度比螢光蛋白溶液被純化級黏土吸附/插層量高出3.18倍。同樣地，也顯示有機化改質之黏土有助紅螢光蛋白吸附/插層於黏土層間。
從XRD、FTIR光譜及TGA之檢測分析來看，螢光蛋白已成功插層於改質黏土(MCL-42)當中。螢光蛋白插層於改質黏土，代表此層間空間提供蛋白質更大的容納空間，且其高層間距亦可作為蛋白質進入其層間之通道，使得蛋白質插層黏土發生的機會提高。

關鍵字

黏土 ； 吸附 ； 螢光蛋白 ； 插層 ； 螢光 ； 改質劑

並列摘要

In this study, the clay, intercalated by cocamidopropylhydroxysultaine, was used to improve the adsorption of DsRed protein. The enhancement of the adsorption of DsRed protein were influenced by the kind of diluted water, pH condition of modified clay, and the concentration of the protein. The optimized process conditions of the protein adsorption has shown when the protein was intercalated/adsorbed by modified clay, preintercalated with cocamidopropylhydroxysultaine (C-50) in pH=7~8 and RO water solvent. The intercalation/adsorption of the protein was highly enhanced from 0.1083 mg/ml to 0.3448 mg/ml when the purified clay was treated by C-50, compared with the purified clay. The result shows that the protein, DsRed, was successfully intercalated/adsorbed in the gallery of clay. There were evidences made by XRD, TGA and FTIR analysis.

並列關鍵字

adsorption ； clay ； fluorescence ； intercalation ； modified agent ； protein