毛細管電泳/紫光LED誘導螢光偵測法

Determination of Dopamine in Urine by Capillary Electrophoresis-Violet Light Emitting Diode-

黃宣銘(Hsuan-Ming Huang)

指導教授 ： 林震煌

國立臺灣師範大學/理學院/化學系/碩士(2003年)

摘要

本研究以紫光/發光二極體（波長410 nm, 2 mW）為螢光激發光源，配合毛細管電泳-線上掃集濃縮技術/誘導螢光偵測法，成功偵測到尿液中微量的多巴胺。這方法是藉由NDA (naphthalene-2,3-dicarboxaldehyde)做為螢光標識試劑，與多巴胺進行衍生反應以後，以螢光偵測的方式進行測量。研究結果發現，以SDS（十二烷基磺酸鈉）為界面活性劑的毛細管微胞電動層析(MEKC)與掃集-毛細管電泳法(sweeping-MEKC)進行分離時，電泳結果所得到的譜峰其理論板數分別為1 × 105、2 × 105；偵測極限為9.1 × 10-7 M與1.2 × 10-8 M。若以sweeping-MEKC的分離模式，且在樣品基質(sample matrix)與背景緩衝溶液(background electrolyte)之間，添加一截甲醇區段，不僅可以延長樣品進樣的體積，使偵測極限到達4.7 × 10-9 M，SDS微胞被甲醇層先破壞後重組的過程之後，理論板數甚至能增加到 1 × 106。

關鍵字

多巴胺 ； 紫光發光二極體

並列摘要

Uses and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method are described. Dopamine labeled-NDA (naphthalene-2,3-dicarboxaldehyde) was selected as the model compound; this new method is also applied to the determination of dopamine in actual urine samples. An inexpensive violet light-emitting-diode (LED) was used for the light source because this is suit for fluorescence excitation. The number of theoretical plates of the analyte was determined to be ~ 1 × 105 and ~ 2 × 105 by means of a MEKC and sweeping-MEKC and this was improved to ~ 1 × 106 when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 × 10-7 M and 1.2 × 10-8 M by means of MEKC and sweeping-MEKC and this was improved to 4.7 × 10-9 M when the methanol plug assisted sweeping-MEKC mode was applied.

並列關鍵字

dopamine ； violet light emitting diode

參考文獻

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