A laser scanning confocal microscope was developed in this study to examine microstructures without incurring the high cost of purchasing a microsope. The confocal microscope uses a 473 nm laser as the light source and employs a high numerical aperture objective (NA = 0.8).It can be used to examine metallic samples with a 200 nm resolution and fluorescence samples with a 100 nm resolution. The confocal microscope is operated with a program coded in LabVIEW™. The program integrates optical elements with the positioning stage, which is connected to the computer. In addition, a preliminary program that restores objects and has a point spread function was developed in this study by using MATLAB®.