磁性螢光奈米複合微粒應用於鹼性磷酸酶與Oct4之檢測及標記

Sensing and labeling of alkaline phosphatase and octamer4 by magnetic optical composite nanoparticles

金煒迪(Wei-Ti Chin)

指導教授 ： 李玫樺

共同指導教授 ： 林宏殷(Hung-Yin Lin)

義守大學/理工學院/材料科學與工程學系/碩士(2014年)

https://doi.org/10.6343/ISU.2014.00228

摘要

近年來誘導型多潛能性幹細胞(iPSC)為一被關注之重點，並應用於再生醫學領域中，如組織修復等。本研究以共沉澱法合成出磁性粒子，並結合拓印高分子技術與氧化鋅螢光粒子，合成出一磁性螢光奈米複合微粒，再以穿透式電子顯微鏡(TEM)、X光繞射儀(XRD)、螢光分光光度計(PL)、超導量子干涉儀(SQUID)分別進行複合粒子之形貌、晶型、螢光性質、磁通量等性質分析。
合成出之磁性螢光奈米複合微粒，粒徑為108.5±13nm，以激發光為360nm時於510nm時有綠螢光發射光，且具有超順磁性，最後利用磁性螢光奈米複合粒子檢測iPSC分化過程中大量表現的鹼性磷酸酶與（Alkaline phosphatase）與關鍵蛋白Oct4（Octamer4），同時利用粒子對iPSC進行綠螢光標記。

關鍵字

磁性粒子 ； 氧化鋅 ； 分子模板 ； 鹼性磷酸酶

並列摘要

In recent years, induced pluripotent stem cells (iPSC) have brought attention to the potential application of the field of Regenerative Medicine, such as tissue repair. In this investigation, the combination of molecular imprinting technique and fluorescent signaling compartment (zinc oxide) leads to the formation of alkaline phosphatase- and octamer4- imprinting composite nanoparticles with the phase inversion of poly(ethylene-co-ethylene alcohol)s. The characterization of molecularly imprinted poly(ethylene-co-vinyl alcohol) coated zinc oxide/Fe3O4 composite nanoparticles were further analyzed using transmission electron microscopy (TEM), X ray diffraction (XRD), fluorescence spectrophotometer (PL), superconducting quantum interference device (SQUID).
The mean size of magnetic/ZnO composite nanoparticles was 108.5 ± 13nm. The photoluminescence (PL) spectrum of composite nanoparticles, having a green fluorescence with an emission at 510 nm was obtained under the excitation at 360 nm. ALP- and Oct4- MIP composite nanoparticles were applied to detect the concentration of alkaline phosphatase and and Oct4 (Octamer4) using PL, respectively. Finally, iPSC cell have been successfully labeled by ALP-MIP magnetic optical composite nanoparticles.

並列關鍵字

magnetic particles ； zinc oxide ； molecular imprinting ； alkaline phosphatase ； Oct4

參考文獻

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