細菌分離株Pseudomonas sp. Kan 2472之醣脂類 生物界面活性劑鑑定

Identification of Glycolipid Biosurfactant Produced by Bacterium Pseudomonas sp. Kan 2472 Isolate

梁睿宸(Ruey-Chern Liang)

指導教授 ： 許世興 ； 羅致逑

朝陽科技大學/理工學院/應用化學系/碩士(2011年)

摘要

本研究自環境中篩選出12株分離株，經分別培養後以細菌16S rRNA專一性引子對進行聚合酶連鎖反應 (Polymerase chain reaction, PCR)，並將反應後產物進行解序，以NCBI (National Center for Biotechnology Information) BLAST 2.0之程式進行GenBank之查詢與比對，初步判定可能菌屬，再以菌種專一性引子對進行菌種確認。同時分析12株分離株之表面張力測量、乳化指數、溶血性試驗，篩選出生產生物界面活性劑較佳之潛力分離株Pseudomonas sp. (Kan 2472)。最後將Pseudomonas sp. (Kan 2472) 菌增量培養收集上清液，以二氯甲烷萃取生物界面活性劑，並將萃取後產物以MALDI-TOF MS進行分析，比對文獻後確認為醣脂類生物界面活性劑Rhamnolipid。

關鍵字

乳化指數 ； 表面張力 ； 生物界面活性劑 ； 溶血性試驗

並列摘要

The objective of present study with 12 selected isolates from the environment, the bacteria were cultured and used for polymerase chain reaction with 16S rRNA-specific primers . By sequencing the reaction products and comparison of GenBank with the program of NCBI (National Center for Biotechnology Information) BLAST 2.0 to preliminary determine the possible genus, then we confirmed the results by species-specific primers. To simultaneous analysis the effects of surface tension measurements, Emulsification index, hemolytic assay, biosurfactant production potential from the isolates, we selected better strain of Pseudomonas sp. (Kan 2472) than the other ones. Therefore we growing cultured and collected supernatants by Pseudomonas sp. (Kan 2472), then used dichloromethane to extract biosurfactant, and analysised the product after extraction by MALDI-TOF MS. The sults xhibited that could be the
glycolipid biosurfactant Rhamnolipid by confirming literature from present papers.

並列關鍵字

biosurfactant ； hemolytic assay ； emulsification index ； surface tension

參考文獻

周建良，「醣脂類生物界面活性劑 rhamnolipid 醱酵基質最適化及生產策略之研究」，碩士論文，國立成功大學化學工程學系，臺南(2000)。

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