凝結芽孢桿菌及芽孢桿菌細胞壁吸附玉米赤黴烯酮毒素及脫氧雪腐鐮刀菌烯醇毒素之研究

Studies on the bioadsorptions of zearalenone and deoxynivalenol by the cell walls of Bacillus coagulans and Bacillus subtilis

劉孟哲(Meng-Zhe Lin)

指導教授 ： 陳齊聖 ； 錢偉鈞

朝陽科技大學/理工學院/應用化學系/碩士(2011年)

摘要

本研究目的主要為探討以Bacillus coagulans及 Bacillus subtilis細胞壁當作吸附劑，吸附玉米赤黴烯酮毒素( ZON )及脫氧雪腐鐮刀菌烯醇毒素( DON )之吸附效果，並觀察不同培養條件，pH 對吸附效果之影響。細胞在不同階段(初生細胞，成長細胞，老細胞)，細胞表面之結構不同，毒素吸附效果因而有所不同。細胞所處環境也可能影響毒素分子之吸附。為瞭解吸附效果， 對於這些影響因子本研究進行系統性之觀察。pH 對於微生物之生長與生化行為常有顯著之影響，而本研究觀察，新生的細胞在pH 4 吸附效果較好，而成熟的細胞，都在pH 5.5、pH 6.5吸附效果較好。
本研究亦觀察到新生細胞吸附效果較差 ( ZON吸附率為34至67 %；DON吸附率為8至37 % )，相對地，老化細胞吸附效果較佳( ZON吸附率為78至81 %；DON吸附率為11至61 % )。這些實驗數據顯示吸附效果因毒素分子特性而異，也受到環境( pH )與細胞種類及狀態之影響。
以本研究所探討之菌株做為飼料添加益生菌，除了提供抑制害菌提昇養分利用率之功能外，同時可降低黴菌毒素之風險，提供動物多一層健康保障。

關鍵字

黴菌毒素 ； 益生菌 ； 吸附劑

並列摘要

This research investigated the bioadsorption of Zearalenone (ZON) and Deoxynivalenol (DON) using the cell walls of Bacillus coagulans and Bacillus subtilis as the bioadsorbent. The effects of cultural condition and pH on the bioadsorptions of the mycotoxins were studied. Cell walls at different (young, matured and old cells) the adsorption effects were found to be different, due to variations of the surface structures of the cells. The pH of the medium is a factor significantly affects the growth and biological behaviors of microbes. Young cells bound the toxins better at pH 4, while for matured cells, the bindings were better between pH 5.5 and 6.5. It was also found that old cells’ binding efficiency (ZON, 78-81%; DON, 11-61%) was better than young cells’ (ZON, 34-67%; DON, 8-37%). Toxin binding was found to be strain dependent. The strains studied in this research, if used as additives of animal feed, have the potential of providing extra health protection by lowering mycotoxin risks besides functioning as probiotics.

並列關鍵字

Mycotoxin ； Probiotics ； Binder

參考文獻

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