摘要
青春痘是人類常見的皮膚問題，當皮脂阻塞堆積於毛囊時，丙酸桿菌便會快速的繁殖，它們所產生的化學物質會使毛囊及其周圍發炎。丙酸桿菌的抗菌物質可抑制其生長，因此可以治療青春痘。在開發抗菌物質的過程常常需要做丙酸桿菌的定量，例如生菌數的測定，然而丙酸桿菌在人工合成的洋菜平板培養基上很難生長，因此開發使丙酸桿菌容易增殖的洋菜培養基是很重要的。 本研究主要是將丙酸桿菌加入芽胞桿菌培養基 (clostriadial medium)、乳酸菌MRS以及新鮮豬肉汁培養基（豬肉汁加一半濃度的LB培養基）等三種不同的平板培養基，在相同的實驗條件下，放置於厭氧缸中，厭氧狀態下，於35℃培養14天，找出丙酸桿菌的最佳培養基。 經由實驗結果得知, 新鮮豬肉汁培養基是丙酸桿菌最佳的培養基。
並列摘要
The problem of acne in human skin is common. It is because propionibacteria grow in the sebum accumulated on the sebaceous unit, forming an inflamentary disorder of skin. The propionibacteria- inactivation substances may be used for the treatment of acne. During the investigation of such antimicrobial substances, the determination of viable counts of propionibacteria is important. However, propionibacteria grow very poor on commercially synthetic agar media. Therefor, the development of an agar medium for the well proliferation of propionibacteria is significant. In the present study, Propionibacterium sp. was cultured in three agar media including clostriadial medium, lactobacilli MRS broth and freshly prepared meat extract medium（meat extract plus half concentration of LB broth）in an anaerobic jar at 35C for 14 days. The growth of propionibacteria cells on each agar plate was evaluated. It was found the freshly prepared meat extract medium was the best medium for cultivation of Propionibacterium sp.