不同萃取溶劑對餘甘子抗氧化性、澱粉水解酶抑制活性及糖化最終產物形成之影響

Effects of Different Extraction Solvents on Antioxidant Activities, Starch Hydrolase Inhibitory Activities and Advanced Glycation End Products Formation of Phyllanthus emblica Linn

江文德(Wen-Dee Chiang) ； 劉上詠(Shang-Yung Liu) ； 高馥君(Fuh-Juin Kao)

《臺灣農業化學與食品科學》 58卷6期 (2020/12) Pp. 163-175

https://doi.org/10.6578/TJACFS.202012_58(6).0004

摘要

本研究在比較沸水、50%甲醇、50%乙醇、95%甲醇、95%乙醇與乙酸乙酯等六種餘甘子萃取物對其自由基清除活性（DPPH、超氧陰離子、NO）、脂肪過氧化抑制活性、澱粉水解酶（α-澱粉酶、α-葡萄糖苷酶）抑制活性及糖化最終產物（AGEs）形成抑制活性之影響，另外也分析各萃取物中活性成份含量。結果發現，以50%甲醇及50%乙醇萃取物的收率、總類黃酮含量與可水解單寧含量最高，而95%甲醇及95%乙醇萃取物的總酚含量、NO清除活性則是最高。所有甲、乙醇萃取物在DPPH自由基清除活性、脂肪過氧化抑制活性、α-葡萄糖苷酶抑制活性與AGEs抑制活性上，皆顯著高於沸水及乙酸乙酯萃取物。HPLC分析結果顯示，沒食子酸苷在六種萃取物中的含量皆是最高，尤以乙酸乙酯萃取物中的含量最豐富。除此之外，所有醇類萃取物中，以50%甲醇及50%乙醇萃取物，具高抑制AGEs形成活性、高α-葡萄糖苷酶抑制活性與較溫和的α-澱粉酶抑制活性，上述的結果指出50%甲醇及50%乙醇萃取物具有潛力開發為營養保健品，用於管理第二型糖尿病。

關鍵字

余甘子 ； 可水解單寧 ； 抗氧化活性 ； 澱粉水解酶 ； 糖化最終產物

並列摘要

The study was to compared the effects of different solvents (boiling water, 50% methanol, 50% ethanol, 95% methanol, 95% ethanol and ethyl acetate) extraction of Phyllanthus emblica Linn. on their scavenging activity of DPPH radical, superoxide radical and NO as well as inhibitory activity of lipid peroxidation, starch hydrolase (α-amylase, α-glucosidase) and advanced glycation end products (AGEs) formation. Furthermore, active compounds of each extract were also determined. The results showed that extracts of 50% methanol and 50% ethanol had highest yield, total flavonoid content and hydrolyzable tannin content, while extracts of 95% methanol and 95% ethanol exhibited the maximum total phenolics content and NO scavenging activities. All the extracts of methanol/ethanol exhibited superior DPPH radical scavenging activities as well as inhibitory activities of lipid peroxidation, α-glucosidase and AGEs formation than extracts of boiling water and ethyl acetate. Based on HPLC analysis, all the extracts, especially ethyl acetate extract, were rich in β-glucogallin. In addition, all methanol/ethanol extracts, especially extracts of 50% methanol and 50% ethanol, showed strong inhibitory activities of AGEs formation and α-glucosidase but mild inhibitory activity of α-amylase. The results indicate that extracts of 50% methanol and 50% ethanol have the potential to be used as nutraceutical in the management of type 2 diabetes.

並列關鍵字

Phyllanthus emblica Linn. ； Hydrolyzable tannin ； Antioxidant activity ； Starch hydrolase ； Advanced glycation end products