This study aimed at determining the effects of eight weeks of plyometric training on the neuromuscular activation of the lower extremities and athletic performance of pre-pubertal boys. Twenty-six elementary school boys aged 10-12 years participated in the study (control group: N = 13; training group: N = 13). The training group underwent low-to-moderate-intensity plyometric training three times a week for eight weeks. Before and after the training, four athletic performance tests, namely height of drop jumps, 20-m sprints, standing hops, and an agility test; and electromyographic assessment of the vastus lateralis, biceps femoris, and gastrocnemius during drop jumps, were conducted. After the training, other than the agility test, the performance tests revealed improvement. The drop jump height, standing hop distance, and 20-m sprint time improved by 17.0% ± 9.7%, 10.6% ± 5.2%, and -4.1% ± 1.9%, respectively. Muscle activation in the vastus lateralis and gastrocnemius during the preparatory phase improved in the training group. During the reactive phase, the vastus lateralis, biceps femoris, and gastrocnemius of the training group revealed an increased activation of 98.84% ± 143.89%, 96.65% ± 117.59%, and 84.14% ± 75.38%, respectively. In conclusion, plyometric training enhanced the athletic performance and neuromuscular function of the children.