鳳凰蟲在不同生長期之代謝體研究

黑水虻屬於水虻科昆蟲的一種，幼蟲期稱為鳳凰蟲，平常以動物糞便或廢棄廚餘維生，其體內富含豐富的動物性蛋白，可以用來生產高品質的動物性飼料。鳳凰蟲本身具有繁殖快速、雜食性但不與人爭食、飼養容易且成本較低的優點，證明它是一個生物轉化的良好媒介。本研究中是利用液相層析串聯質譜技術（LC-MS）與統計分析方法（PCA, OPLS-DA）相結合，探索鳳凰蟲在不同生長期下代謝物的變化。實驗可分為兩個部分，第一部分是芝麻成分的分離純化，探討芝麻成分中主要被鳳凰蟲利用的是什麼。第二部分是代謝物差異分析，探討0期之空白組與2期、4期、5期之實驗組鳳凰蟲的體內成分差異，以及2期、4期、5期鳳凰蟲之間糞便的代謝差異分析。根據實驗數據結果表示，在芝麻中主要被利用的成分為RT = 26 min，經過open column的分離純化與半製備，可以由NMR的結果推測RT = 26 min為甘油二酯。代謝物差異中，分子量278在鳳凰蟲體內及糞便中都有明顯的變化，推測此成分為鳳凰蟲整個生長過程極為重要的成分。透過了解鳳凰蟲在不同生長期下代謝的變化，可以瞭解其體內生物轉換的機制，未來期許能夠把此成果應用在新藥之生物技術發展方面，盡自己一份心力。

關鍵字

黑水虻；代謝體學；液相層析串聯式質譜儀；統計分析

並列摘要

Hermetia illucens L. (HI), the species of Stratiomyidae insects, which lives by livestock manure and kitchen waste. The abundance of protein can be used to produce high-value animal protein feed. In addition, there are many advantages, i.e., reproduce rapidly, euryphagous except human food, raise easily and low cost. Based on the above, HI is a good media for resource transformation. In this study, we applied LC-MS technique combined with statistical analysis (PCA, OPLS-DA) to explore the different growth stages of HI. The growth stages of the larvae are five, the freshly hatched larvae were sacrificed and freeze-dried as the blank of zero stage. The rest of larvae were reared and fed on Sesamum indium (SI), and they would enter the next stage by each peeling. We took the second, fourth and fifth stages by each peeling a week for analysis. According to the LC-MS data, we run the database software-Compound discoverer 2.0 to get predicted compounds information like retention time, chemical formula, and area under the curve. After we get these information, we also run statistical software-SIMCAP to analysis the difference among SI and HI, HI with fed SI. In summary, the results from LC-MS technique combined with statistical analysis can be speculated that the use of energy insects Hermetia illucens L. is not only amino acids, but also free fatty acids.

並列關鍵字

Hermetia illucens L. ； Metablomics ； LC-MS ； statistical analysis

參考文獻

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鳳凰蟲在不同生長期之代謝體研究

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