小球藻於不同培養條件下之木薯廢水資源化與減排研究

微藻可藉由光合作用固定大氣中之二氧化碳並將其轉換成生物質，因其生長快速、土地需求面積小，被視為是一種具潛力的生物質原料，但目前所面臨最大的問題在於其培養基相當昂貴。木薯廢水中含有大量有機物質及營養鹽需要被去除，根據許多研究中皆指出微藻可藉由光合作去除水中營養物質，本研究試圖結合小球藻與木薯澱粉廢水以達成廢水之資源化與減排。　　本研究以單一變數法探討廢水預處理、藻體預培養、廢水濃度及投入藻量對於微藻生物質濃度影響，並以回應曲面法探討不同照度、廢水濃度及微藻投入量對於微藻及水體之影響。根據結果藻體在培養條件為照度為6000 lux、投入小球藻之O.D.值為0.1、廢水濃度為0.15倍的情況下進行培養，可獲得最高之比生長速率2.05 day-1。當照度為6000 lux、投入PA藻之O.D.值為2、廢水濃度為0.15倍的情況下進行兩天的培養便可同時去除75%之COD、78%之蛋白以及85%的總磷。　　應用層面而言，12:12小時光暗週期及以廢水作為預培養基皆是可行的。而相較於批次培養，在每日替換率為0.1至0.15之半連續式培養，在8天內則可獲得更高的藻體濃度及穩定的COD排放值。

關鍵字

木薯廢水；小球藻

並列摘要

Microalgae can convert carbon dioxide into biomass as a result of their photosynthetic activity. It is kind of potential raw material of biomass energy because it’s high growth rate and low land resource for cultivation. However, the most serious problem is that the microalgae culture medium is very expensive. The photosynthesis of microalgae can remove organic and inorganic nutrients in cassava wastewater before discharging to natural water environment. The wastewater was used as culture medium for Chlorella to culture microalgae and reduce the pollutant in the wastewater. 　　The single variable method was used to check whether the pre-culture medium, wastewater pretreatment, wastewater concentration and O.D. value is important for this research. And the response surface method was used to investigate the effect of illuminance, initial O.D. value and wastewater concentration. While 6000 lux of illuminance, 0.1 of initial O.D. value and 0.15X of wastewater concentration have the highest specific growth rate 2.05 day-1. 75% of COD, 78% of protein and 85% of TP can be removed under 6000 lux of illuminance, 2 of initial O.D. value and 0.15X of wastewater concentration after two days of culture. 　　For the application of microalgae, the cassava wastewater can used as pre-culture medium for Chlorella and culture under 12:12 light/dark cycle. Higher concentration of Chlorella and stable COD of wastewater can be achieved by using semi-continuous reactor under dilution rate between 0.1 to 0.15 during eight days of culture.

並列關鍵字

cassava wastewater ； Chlorella

參考文獻

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小球藻於不同培養條件下之木薯廢水資源化與減排研究

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