In this thesis, we report synthesis and experimental studies of photophysical and photochemical properties of deuterated distyrylbenzene (DSB) isomers in which the hydrogen atoms in the vinyl positions of DSB are replaced by deuterium atoms. We have assigned vibrational modes of these DSB isomers and their deuterated components based on infrared absorption and Raman measurements. Absorption and fluorescence properties, including fluorescence lifetime and quantum yield, have been investigated in great detail. We discovered that no distinct difference was observed between non-deuterated and deuterated compounds. Kinetic measurements of these deuterated DSB isomers have yielded distinguishable difference in photoisomerization rate compared to non-deuterated isomers. Kinetic models were proposed to explain these behaviors and comparison was made with the case of stilbene.