Title

運動訓練對癌症小鼠模式腫瘤生長及惡病質之影響

Translated Titles

Effects of Exercise Training on Tumor Growth and Cancer Cachexia in Tumor-Bearing Mice Model

Authors

蔡孟書

Key Words

癌症惡質 ; 肌肉生長抑制素 ; 肌肉萎縮 ; 腫瘤生長 ; 血管內皮生長因子 ; cancer cachexia ; myostatin ; muscle wasting ; tumor growth ; vascular endothelial growth factor-A

PublicationName

臺灣大學物理治療學研究所學位論文

Volume or Term/Year and Month of Publication

2013年

Academic Degree Category

博士

Advisor

吳英黛

Content Language

英文

Chinese Abstract

肺癌的死亡率高,存活者常伴隨體重顯著下降,目前已知腫瘤的生長及轉移會受到血管新生(angiogenesis)及血管內皮生長因子(vascular endothelial growth factor A, VEGF-A)影響,而肌肉量降低可能與肌肉生長抑制素(myostatin)增加有關。運動訓練有助於肺癌病患改善運動耐力及生活品質,在一些癌症動物模式中,運動可以減緩腫瘤生長,也有報告可以降低正常人肌肉生長抑制素表現,然而運動訓練對肺癌模式腫瘤生長及肌肉量降低的研究罕見。 本系列研究目的在探討運動對肺癌模式小鼠腫瘤生長及惡病質之影響,同時也將測量血管內皮生長因子A與肌肉生長抑制素表現。研究一在建立並探討Lewis肺癌模式小鼠的特徵,並與正常小鼠比較其血清的血管內皮生長因子A變化。研究二是探討運動訓練對癌症小鼠模式腫瘤生長及血管內皮生長因子A的影響。研究三的目的則是探討運動訓練對癌症小鼠模式腫瘤生長及惡病質的影響,以及對肌肉生長抑制素及titin cap的影響。 研究一的腫瘤模式小鼠在第35天的存活率為56.3%,腫瘤體積為4264.69 ± 1038.32 mm3,腫瘤重量為3.70 ± 0.83 g。相較於正常組小鼠,其體重增加幅度較少、肝臟(2.22 ±1.20)與肺臟(6.33±2.51)有腫瘤轉移結節且血清的血管內皮生長因子A濃度明顯上升。 研究二進一步發現運動訓練會增加腫瘤模式小鼠血清血管內皮生長因子A,但對腫瘤生長及腫瘤重量沒有影響,控制組之腫瘤重量為3.70±0.83 g (n=9),運動訓練組則為4.64±1.12 g (n=10)。 研究三我們以較少的Lewis肺癌細胞探討運動訓練對早期癌症惡病質之影響。證實有氧運動訓練可以降低腫瘤重量(腫瘤控制組為2.94±0.45 g、腫瘤運動訓練組為1.87±0.23 g)及肝臟重量。運動訓練可以增加腓腸肌與股四頭肌相對於非腫瘤重量比值,然而腫瘤訓練組與腫瘤控制組之肌肉量與橫膈肌的肌纖維截面積沒有差異。此外,腫瘤組腓腸肌的肌肉生長抑制素及titin cap及橫膈肌的肌肉生長抑制素受器activin receptor type IIB(ACTRIIB)表現量上升。 本研究論文建立Lewis肺癌模式小鼠的腫瘤生長與癌症惡病質現象,發現四週運動訓練會增加腫瘤運動組血清中的血管內皮生長因子A,但是對腫瘤生長與存活率沒有影響。且運動訓練可以降低早期Lewis肺癌模式小鼠腫瘤重量及減緩惡病質。

Topic Category 醫藥衛生 > 醫藥總論
醫學院 > 物理治療學研究所
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