This research adopt some optimizationmodels and stoichiometric matrix to formulate the problem of overproducing alcohol and lactic acid in microbial system. Stoichiometric matrix is useful for describing the flux balance and optimization model determines the efficiency of manipulation reactions. At the same time, some biological constraints have to be included into the optimization problem, and we can get promising results which are close to real bio-systems. Consequently, we find the optimal solution accomplishing the maximum solution of biomass yield. Most strategies can get more than 90% of the maximum theoretical yield, and the flux distribution can be also determined. We also find that the value of the regulation strength parameter would affect the production yield. When the value of regulation strength parameter increases, we can get a higher yield. The proposed strategies are applied to batch reactor, and the performance of the bacteria could be observed.