Title

超解析率廣視野螢光顯微系統於細胞核膜形貌研究之應用

Authors

黃旭成

Key Words

超解析

PublicationName

中正大學物理學系學位論文

Volume or Term/Year and Month of Publication

2014年

Academic Degree Category

碩士

Advisor

林俊元

Content Language

繁體中文

Chinese Abstract

廣視野螢光顯微術在生物影像觀測上為一重要量測工具,本系統另外加入二維結溝光照明後突破繞射極限,大幅提高解析度,也具有良好的切片能力,因此在觀測生物樣品時能夠非侵入式的量測,不破壞樣品本身的結構。 在此次的研究中,以本實驗技術觀察真實生物樣品,藉由超高影像解析度分辨蛋白質之間的相對位置,提供資訊幫助生科所的團隊能夠近一步了解蛋白質對人體的影響。 經過量測,系統橫向解析度為136 nm,縱向解析度約為400 nm。而在拍攝標準生物樣品的時間僅需10秒。經過程式處理得到超高解析度影像只需6秒。而在長時間的拍攝生物樣品時,系統的定位準確度可達1pixel。 而在厚樣品的處理上,使用立體疊圖的方式來觀測細胞核膜邊界的形貌,藉以提高辨識度。

Topic Category 基礎與應用科學 > 物理
理學院 > 物理學系
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