應用修飾螢光分子之中孔洞二氧化矽奈米粒子進行癌細胞內酸鹼值偵測與三維空間即時追蹤

鄒劼叡

doi:10.6342/NTU.2014.02253

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應用修飾螢光分子之中孔洞二氧化矽奈米粒子進行癌細胞內酸鹼值偵測與三維空間即時追蹤

Utilizing Fluorescent Dye Loaded Mesoporous Silica Nanoparticle for pH Sensing and 3D Real Time Tracking in Cancer Cell

鄒劼叡 , Masters　　Advisor：牟中原　　

英文 DOI： 10.6342/NTU.2014.02253

中孔洞二氧化矽；癌細胞；酸鹼值偵測器；三維空間即時追蹤；胞吞作用； Mesoporous silica ； Cancer cell ； pH sensor ； 3D Real Time Tracking ； Endocytosis

Abstract │ Reference (125) │ Altmetrics

Abstract 〈TOP〉

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Intracellular pH plays many important roles in cell, including proliferation, apoptosis and endocytosis. Therefore, information of intracellular pH can help us to understand the cellular dysfunctions and the physical conditions of organelles. Since the discovery of mesoporous silica nanoparticle (MSN), such silica materials with controllable physical properties and excellent biocompatibility have received tremendous attention. Because the silanol groups on surface are available for further modification of diverse functional groups, MSN has been employed in drug delivery, intracellular imaging and sensing. 
Herein, dye-leaded MSN is synthesized by co-condensing a pH-sensitive dye FITC and a reference dye RITC with silica source. By ratiometric method, one can correctly obtain the readout of this pH sensor. In the first part of the thesis, we demonstrate a 3D real time tracking technique which can record the trajectories of the fluorescent dye-loaded MSN (FMSN) internalized by cancer cell and the change of pH at the same time. The motion of FMSN and pH are proved highly correlative by observing the acidification after endocytosis and the increase of pH due to endosome escape. Moreover, the cellular dysfunction is shown responsible for the occurrence of endosome escape. In the second part, we synthesize hollow structured FMSN by adding hexane during the synthesis, which also leads to a broad pore size distribution. The different surface curvature due to various pore sizes affects not only the distance between FITC and positive-charged functional groups on the silica surface but the pKa value of FITC. As a result, we obtain a powerful pH sensor which owns a broad pH sensing range. We envision these researches can open up new designs of sensors and improve the knowledge of cellular process.

Reference ( 125 ) 〈TOP〉

1. Kresge, C. T.; Leonowicz, M. E.; Roth, W. J.; Vartuli, J. C.; Beck, J. S. Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism. Nature 1992, 359, 710-712.
連結：
2. Nooney, R. I.; Thirunavukkarasu, D.; Chen, Y.; Josephs, R.; Ostafin, A. E. Synthesis of Nanoscale Mesoporous Silica Spheres with Controlled Particle Size. Chem. Mater. 2002, 14, 4721-4728.
連結：
3. Lin, H.-P.; Mou, C.-Y. Structural and Morphological Control of Cationic Surfactant-Templated Mesoporous Silica. Acc. Chem. Res. 2002, 35, 927-935.
連結：
4. Zhao, D.; Huo, Q.; Feng, J.; Chmelka, B. F.; Stucky, G. D. Nonionic Triblock and Star Diblock Copolymer and Oligomeric Surfactant Syntheses of Highly Ordered, Hydrothermally Stable, Mesoporous Silica Structures. J. Am. Chem. Soc. 1998, 120, 6024-6036.
連結：
5. He, Q.; Zhang, Z.; Gao, Y.; Shi, J.; Li, Y. Intracellular Localization and Cytotoxicity of Spherical Mesoporous Silica Nano- and Microparticles. Small 2009, 5, 2722-2729.
連結：

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