應用幾丁質及奈米複合顆粒於幾丁聚醣酶與蛋白酶之純化

陳政融

doi:10.6846/TKU.2011.00692

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應用幾丁質及奈米複合顆粒於幾丁聚醣酶與蛋白酶之純化

Application of chitin particles on the purification of chitinolytic enzymes and proteases

陳政融 , Masters　　Advisor：王三郎　　Co-advisor ：薛文發

繁體中文 DOI： 10.6846/TKU.2011.00692

蛋白酶；幾丁質酶；磁性幾丁聚醣/四氧化三鐵納米複合顆粒；粗製蝦殼幾丁質； Protease ； chitinase ； Magnetic chitosan / Fe3O4 nanocomposite particles ； Crude SSC

Abstract │ Reference (22) │ Altmetrics

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Application of NF and Crude SSC on the purification of enzymes to achieve rapid purification purposes. Adsorbent : SP, AC, pure chitin, Crude SSC, Crude CSC, Crude SPC , Choose a more suitable adsorbent Crude SSC. Studying temperature, weight, time on the adsorption effect of the pineapple enzyme. By experiment that at 4 ℃, 30 min, 0.1 g of the conditions to get specific activity 0.0918 U / mg.
  Studying temperature, weight, time on the effect of NF adsorption bromelain enzyme. Learned from the experiment at 25 ℃, 30 min, 0.1 g of the conditions to get specific activity 0.1357 U / mg.
  Use the above conditions, NF, and Crude SSC absorption bacterial enzyme purified enzyme to discuss the results. Purified enzyme affected by the experimental conditions known isoelectric point is not the only factor, but also need to consider the interaction between enzyme and adsorbents as well as physical absorption and other factors.
  Results show that absorption is a viable way to purify the enzymes, it can improve the purification efficiency.

Reference ( 22 ) 〈TOP〉

Alexandre-Franco M, Fernández-González C , Alfaro-Domínguez M, Gómez-Serrano V (2011) Adsorption of cadmium on carbonaceous adsorbents developed from used tire rubber. Journal of Environmental Management ,92 :2193-2200.
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Dotto G L, Pinto L A A (2011) Adsorption of food dyes onto chitosan: Optimization process and kinetic. Carbohydrate Polymers, 84 :231–238.
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Fajardo P, Martins J T , Fucinos C , Pastrana L, Teixeira J A, Vicente A A (2010) Evaluation of a chitosan-based edible film as carrier of natamycin to improve the storability of Saloio cheese . Journal of Food Engineering, 101 : 349–356
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Gupta B, Arorab A, Saxenaa S , Alam M S (2008) Preparation of chitosan–polyethylene glycol coated cotton membranes for wound dressings: preparation and characterization, Polymers for Advanced Technologies, 20: 58–65.
連結：
Harish Prashanth K V ,Tharanathan R N (2007) Chitin/chitosan: modifications and their unlimited Application potentialdan overview. Trends in Food Science & Technology 18:117e131
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