直接大氣壓下化學游離質譜法應用於蜂蜜不同蜜源及產地之鑑定

吳宗元

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直接大氣壓下化學游離質譜法應用於蜂蜜不同蜜源及產地之鑑定

Identification of Botanical and Geographic Origins of Honey Using Direct APCI-MS Analysis

吳宗元 , Masters　　Advisor：沈振峯　　

繁體中文

氣盾式大氣壓化學游離法；直接分析；揮發性；蜂蜜；主成分分析； Atmospheric Pressure chemical ionization ； Direct analysis ； Volatiles ； Honey ； PCA

Abstract │ Reference (79) │ Altmetrics

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Honey is a sweet natural product produced by Apis mellifera from the nectar of plants (nectar, honey).  Each honey possesses its own aroma and flavor, depending mainly on the botanical sources that contribute to its production. In this study, a simple diret APCI-MS analysis was applied to detect the fingerprinting mass spectra of the honey samlples after 10-fold dilution with water. The obtained mass spectra were found to be useful in the identification of the honey from different botanical and geographic origins. By using the characteristic ions/ion ratios, the mass spectra database (fingerprinting mapping) and PCA strategies, all the idetifications were valid. 30 blind samples were examined with the strategies, the successful ID rates were 29/30 using ion ratio, 28/30 using liberary search and 28/30 using PCA method. The results evidenced the ultilty of the direct APCI-MS analysis for the identification of the honey oringins.

Keywords: Atmospheric Pressure chemical ionization, Direct analysis, Volatiles, Honey, PCA

Reference ( 79 ) 〈TOP〉

[2] A Mizrahi and Y. Lensky., "Bee products: properties, applications, and apitherapy.," 1997.
連結：
[3] C. Nasuti, R. Gabbianelli, G. Falcioni, and F. Cantalamessa, "Antioxidative and gastroprotective activities of anti-inflammatory formulations derived from chestnut honey in rats," Nutrition Research, vol. 26, pp. 130-137, 2006.
連結：
[4] 楊富鈞, "結合蜂蜜花粉分析與高效液相層析鑑定台灣荔枝蜜與龍眼蜜," 2010, 國立臺灣大學生態學暨演化生物學研究所碩士論文。
連結：
[5] I. S. Arvanitoyannis, C. Chalhoub, P. Gotsiou, N. Lydakis-Simantiris, and P. Kefalasb., "Novel Quality Control Methods in Conjuction With Chemometrics (Multivariate Analysis) For Detecting Honey Autenticity," 2005.
連結：
[6] E. Alissandrakis , Petros A. Tarantilis , Paschalis C. Harizanis , and M. P. b., "Aroma investigation of unifloral Greek citrus honey using solid-phase microextraction coupled to gas chromatographic-mass spectrometric analysis," Food Chemistry, vol. 100, pp. 396-404, 2007.
連結：

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