- 期刊
Phylogenetic Relationships of Cordyceps Species Revealed by ITS Sequences of Ribosomal DNA
並列摘要
The internal transcribed spacer (ITS) regions of seven Cordyceps species and two species belonging to the same family of Clavicipitaceae, Phytocordyceps ninchukispora and Claviceps purpurea, were analyzed by polymerase chain reaction amplification and direct DNA sequencing. Phylogenetic relationship trees based on the sequences of ITS-I and ITS-II were constructed. The sequence similarities of the two regions of C. sinensis between the fresh samples collected from Tibet and the dried specimens from Chinese herb stores in Taiwan were 99.5% and 99.7%, respectively. However, the sequences were compared with those of C. sinensis collected from the CCRC (Culture Collection and Research Center). The similarities of ITS-I and ITS-II were only 71.2% and 81.5%, respectively. Equivalent sequencing results were obtained no matter whether the genomic DNA was extracted from the tissues of C sinensis, its stroma, or the host of the fungus.
被引用紀錄
延伸閱讀
- 孫舜國、楊淳翔、陳淑德（2018）。Effects of Light on Growth and Metabolite Production of Cordyceps militaris。農林學報，66(2)，117-128。https://doi.org/10.30089/JAF.201806_66(2).0005
- 趙書慶（1998）。Identification of fungi in the genus Cordyceps by ribosomal DNA internal transcribed spacers (ITS)〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714264758
- 丘錦朋（2011）。The culture, identification and bioactivity analysis of Cordyceps militaris〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0003-0808201116071700
- 梁宗琦（1997）。Biodiversity of the Genus Cordyceps and its Anamorphs。Fungal Science，12(1&2)，51-57。https://doi.org/10.7099/FS.199706.0051
- Chan, W. H., Ling, K. H., Chiu, S. W., Shaw, P. C., & But, P. P. H. (2011). Molecular Analyses of Cordyceps gunnii in China. 藥物食品分析, 19(1), 18-25. https://www.airitilibrary.com/Article/Detail?DocID=10219498-201103-201104200015-201104200015-18-25