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Electrophysiological Interactions of Acetylcholine, Norepinephrine and 5-Hydroxytryptamine in the Rat Hypothalamic Slice
Acetylcholine, Norepinephine及5-Hydroxy tryptamine於大白鼠離體腦下視丘前部感溫細胞之電生理作用
摘要
本研究探討大白鼠離體腦下丘視前部感溫細胞對acetylcholine， norepine-phrine及5-hydroxytryptamine（5-HT）的電生理反應。此區的細胞可分為感熱、感冷及非感溫細胞。我們發現將acetylcholine當以多管微電極及微量電流注射法，直接置於下視丘前部感溫細胞，多為興奮性作用。雖然norepinephrine對多數的感冷細胞之作用與acetylcholine相似，其對感熱細胞則呈抑制作用。5-HT對感溫細胞之作用與norepinephrine相反。這些發現證明了在離體下，腦細胞仍具備副交感及單胺神經傳導。此外本結果對在無麻醉及神經導入訊號下，影響體溫的生理及藥理因素，提供了進一步的了解。
並列摘要
The purpose of this experiment is to investigate the electrophysiological properties of the thermosensitive neurons in the hypothalamic brain slice. Hypothalamus was dissected and was sliced from adult male Sprague-Dawley rats. Extracellular neuronal activity was recorded from a single neuron of preoptic/anterior hypothalamic (POAH) area of diencephalon from the slice. Cells were classified into warm-sensitive, cold-sensitive, and thermally insensitive groups. We found that acetylcholine, applied locally through a multibarrel micropipette by iontophoresis, excited most of POAH neurons. On the other hand, NE inhibited most of warm-sensitive neurons and excited cold-sensitive neurons. Interestingly, the effects of 5-HT were opposed to that induced by NE. In conclusion, our data suggested the cholinergic and monoaminergic transmission were preserved in the in vitro environments.
延伸閱讀
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