The subpopulation of leukocytes can divide into PMN, monocyte and lymphocyte. These cells are all human body's important immune systems of defence. If the immune cells are in-juried by oxidative stress, it may be unable to work normally. Growthing evidences indicated that the intensity during exercise is one of the reasons which changes player's physiological phenomenon. In high intense and acute exercise, because the increase of the aerobic metabolism causes higher oxidants in the body; If the reduced status is unable to balance oxidants, oxidative stress will be produced then contributed to oxidative damage in cells or tissues. The purpose of this study was to investigate the effect of one acute bout of exercise on oxidants and reductants in the subpopulation of leukocytes. Eight endurance runners who performed aerobic exercise for 3 consecutive days (85% VO2max) on treamill and every event for 30 min were recruited in this study. Venous blood samples were collected from subjects running before 12 hr. immediately after. respectively. The levels of hydroxyl peroxide (H2O2), glutathione (GSH) concentration in subpopulation of leukocytes were measured by FACSca1-iber flow cytometer. H2O2 concentration was increasing trend in subpopulation of leukocytes and there were significantly enhanced in monocytes and lymphocytes (p<0.05). Especially, the increasing percentage of oxidants was highest in lymphocytes after acute running. In addition to, GSH concentration was also increasing in subpopulation of leukocytes after running (p<0.05). The higher increasing percentage of reductant was emerged in PMN. Conclusion: After acute and aerobi c exercise may be responsible for higher status of oxidants in subpopulation of leukocytes and reductants exhibited positive response to oxidants. However, the phenomenon of oxidative stress in leukocyte could still probably be produced after acute aerobic exercise.
The subpopulation of leukocytes can divide into PMN, monocyte and lymphocyte. These cells are all human body's important immune systems of defence. If the immune cells are in-juried by oxidative stress, it may be unable to work normally. Growthing evidences indicated that the intensity during exercise is one of the reasons which changes player's physiological phenomenon. In high intense and acute exercise, because the increase of the aerobic metabolism causes higher oxidants in the body; If the reduced status is unable to balance oxidants, oxidative stress will be produced then contributed to oxidative damage in cells or tissues. The purpose of this study was to investigate the effect of one acute bout of exercise on oxidants and reductants in the subpopulation of leukocytes. Eight endurance runners who performed aerobic exercise for 3 consecutive days (85% VO2max) on treamill and every event for 30 min were recruited in this study. Venous blood samples were collected from subjects running before 12 hr. immediately after. respectively. The levels of hydroxyl peroxide (H2O2), glutathione (GSH) concentration in subpopulation of leukocytes were measured by FACSca1-iber flow cytometer. H2O2 concentration was increasing trend in subpopulation of leukocytes and there were significantly enhanced in monocytes and lymphocytes (p<0.05). Especially, the increasing percentage of oxidants was highest in lymphocytes after acute running. In addition to, GSH concentration was also increasing in subpopulation of leukocytes after running (p<0.05). The higher increasing percentage of reductant was emerged in PMN. Conclusion: After acute and aerobi c exercise may be responsible for higher status of oxidants in subpopulation of leukocytes and reductants exhibited positive response to oxidants. However, the phenomenon of oxidative stress in leukocyte could still probably be produced after acute aerobic exercise.