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  • 期刊

Endogenous and Exogenous Contrast Agents of Third-harmonic-generation Microscopy

組織內三倍頻之分子對比與外加顯影劑

摘要


此論文以鉻貴橄欖石雷射做為光源的非線性雷射掃瞄顯微鏡系統探索生物體中仍未被發現的三倍頻對比來源和尚未被使用的三倍頻外加顯影劑,證實來自彈性蛋白纖維的三倍頻對比,和醋酸與蘇木素作為三倍頻顯影劑的可行性。

並列摘要


The purpose of this thesis is to explore other yet undiscovered contrast origins and unused contrast agents of third-harmonic-generation (THG) inside biological tissues, and apply to the preliminary studies of medicine based on a nonlinear laser scanning microscopy system using a femtosecond Cr: forsterite laser centered at 1,230-nm. The scope of this thesis contains that THG contrast from elastic fibers is detected and demonstrated, and using acetic acid or hematoxylin as THG contrast agents is verified to be feasible.

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