同軸電紡絲在傷口敷藥的應用

Application of core-shell electrospinning on wound dressing

朱柏穎(Po-Ying Chu)

指導教授 ： 蔡偉博

國立臺灣大學/工學院/化學工程學研究所/碩士(2011年)

https://doi.org/10.6342/NTU.2011.02441

摘要

現今傷口敷藥有許多形式，電紡絲是其中的一種。電紡絲擁有高孔隙度、高比表面積、孔洞小等優點，另外還可以模仿細胞外間質的構造，所以電紡絲是傷口敷藥的良好選擇之一。本實驗利用同軸電紡絲的技術來製造軸心為聚(乳酸-甘醇酸)而殼層為明膠之電紡絲或者是軸心和殼層都是明膠的電紡絲，其所形成的電紡絲薄膜當作傷口敷藥的生醫材料。
由於明膠對水的溶解度相當高，所以交聯是必須要的。經過交聯過後的電紡絲比沒交連過的電紡絲泡水後重量損失較少。且良好的傷口敷藥必須有較佳的吸水性，所以在本研究中也測試了吸水性，沒有交聯過的聚(乳酸-甘醇酸)-明膠同軸電紡絲有較佳的吸水性，然而用EDC/NHS交聯的吸水性較差；而明膠電紡絲用EDC/NHS有較佳的吸水性。最後，包埋藥物做藥物釋放，沒有經過交聯的聚(乳酸-甘醇酸)-明膠同軸電紡絲在兩個小時內就釋放了將近80%，而經過戊二醛蒸氣交聯的聚(乳酸-甘醇酸)-明膠同軸電紡絲其釋放量大概只有一半左右：而在明膠-明膠同軸電紡絲經過戊二醛蒸氣交聯後，起初其同軸電紡絲釋放量較單軸電紡絲為慢。
本研究成功證實以同軸電紡絲交過戊二醛蒸氣交聯後可有效減少明膠易溶於水的情況，並且可以達到良好的吸水性和控制藥物釋放的結果。

關鍵字

傷口敷藥 ； 同軸電紡絲 ； 聚乳酸-甘醇酸 ； 明膠 ； 藥物釋放

並列摘要

Due to the advance in biomaterial, wound dressing has been fabricated in many forms in recent days, where electrospinning-based material has been known as one of the potential candidates. Since electrospinning consists of advantages due to its high porosity, high specific area, and small pore size, it can mimic the extracellular matrix (ECM) to apply in wound dressing. In this study, core-shell technique was used to fabricate core-shell PLGA-gelatin and gelatin-gelatin fiber meshes to serve as the biomaterial of wound dressing.
Since gelatin consists of high water-solubility, crosslinkage is applied on the fabricated fibers to examine its physical character. Generally, ideal wound dressing should possess great water adsorption; therefore the water capacity of the crosslinked and non-crosslinked fibers was studied. The weight of non-crosslinked fiber meshes were observed to be more than those of crosslinked fiber meshes. However, the water adsorption of non-crosslinked core-shell PLGA-gelatin fiber mesh is shown to be higher than the crosslinked fibers, with even higher degree of water adsorption for fibers that were crosslinked by EDC/NHS. When analyzing the release profile of the fibers, about 80% of the entrapped drugs were released from non-crosslinked core-shell PLGA-gelatin fibers which are twice more than the glutaraldehyde vapor-crosslinked fibers. However, the release from core-shell gelatin-gelatin fiber crosslinked by glutaraldehdye vapor is slower than those of monoaxial gelatin in initial period.
In this study, core-shell electrospinning crosslinked by glutaraldehyde vapor was observed to effectively reduce the weight loss in water with great water adsorption to perform sustained release of drugs.

並列關鍵字

wound dressing ； core-shell electrospinning ； PLGA ； gelatin ； drug release

參考文獻

1. Chong, E.J., et al., Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomaterialia, 2007. 3(3): p. 321-330.

2. Tsuruta, D., et al., The barrier function of skin: how to keep a tight lid on water loss. Trends in Cell Biology, 2002. 12(8): p. 355-357.

3. MacNeil, S., Progress and opportunities for tissue-engineered skin. Nature, 2007. 445(7130): p. 874-880.

5. Muzzarelli, R.A.A., Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 2009. 76(2): p. 167-182.

6. Fonder, M.A., et al., Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings. Journal of the American Academy of Dermatology, 2008. 58(2): p. 185-206.