背景:阿茲海默症主要臨床症狀為記憶能力喪失,其病理特徵為老年斑,而老年斑主因類澱粉樣蛋白(amyloid β-peptide)沉積所致,基於此理論本實驗設計應用amyloid β peptide(Aβ_(1-40))來誘發阿茲海默症學習障礙之動物模型,並以水迷宮來評估中藥牛膝改善學習記憶障礙的作用。材料&方法:本計畫以80% 乙醇及水進行藥材萃取及分離,分別提取純化而製得懷牛膝皂苷提取物(ABS)及懷牛膝多醣體提取物(ABP);後進行對Aβ_(1-40)誘導大鼠記憶障礙改善作用之評估。結果:Aβ_(1-40)可造成大鼠於水迷宮之操作障礙暨短期記憶與參考記憶之障礙。ABS於50 mg/kg可改善Aβ_(1-40)側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙;而ABP於100 mg/kg也能改善Aβ_(1-40)側腦室輸注所導致水迷宮空間操作暨參考記憶能力之障礙。結論:懷牛膝之成分ABS及ABP能改善Aβ_(1-40)誘導大鼠水迷宮學習記憶障礙,可供中醫藥臨床基礎研究參考。
Purpose: Alzheimer's disease (AD) is characterized by neuropathological hallmarks such as senileplaque and neurofibrillary tangles caused by the amyloid β peptide (Aβ_(1-40)). For this reason, we demonstratedthe role of the improve effect of Achyranthesbidentata against continuous intracisternal injection of Aβ_(1-40) in rats. Materials & Methods: This present result obtained total saponins and total polysaccharides extracted from Actyranthes bidentate (ABS and ABP) by 80% ethanol and distilled water. We further investigated the effects of ABS and ABP on the memory function caused by Aβ_(1-40) in rats. Intracisternal injection of Aβ_(1-40) caused the dysfunction of memory function on water maze. Results: Pretreatment with ABS at a dosage of 50 mg/kg or ABP at 100 mg/ kg reversed the dysfunction of memory function on water maze caused by intracisternal injection of Aβ_(1-40). Conclusions: ABS and ABP can improve the learning and memory impairment of rat water maze induced by Aβ_(1-40), which can be used as a reference for clinical basic research of traditional Chinese medicine.