大氣質譜技術應用在快速辨識毒物的潛力

The Potential of Ambient Mass Spectrometry in Rapid Identification of Intoxicating Compounds

李啟偉(Chi-Wei Lee) ； 蘇鴻(Hung Su) ； 謝建台(Jentaie Shiea)

《化學》 76卷1期 (2018/03) Pp. 29-44

https://doi.org/10.6623/chem.201803_76(1).005

摘要

急診室裡其中一種常見之急性中毒案例乃致命的服農藥自殺中毒，但因傳統液相層析質譜技術的樣品前處理太過耗時，不足以應付緊急醫療狀況。針對上述這類毒物之毒性成分，目前已成功開發一高效能大氣質譜快速檢測技術平台，未來對解決急診室中毒性物質辨識效率偏低的問題頗俱潛力。服農藥約佔全球自殺事件的三分之一。為了加快在急診的搶救行動，本研究研發出一種通過熱脫附電噴灑游離質譜法(TD-ESI/MS)來快速鑑定攝取農藥種類的分析方法。本檢測口腔液中農藥的方法其再現性(n = 6)的相對標準偏差(＜ 7%)低，而從四位受試者獲得的口腔液中檢測農藥的極限在1-10 ppb之間，其相對標準偏差為10.7%。五種口腔液中農藥在1 ppb-1 ppm濃度範圍內其與質譜訊號強度間的線性關係良好(R^2在0.9938和0.9988之間)。由於整個分析過程極為快速，這種技術讓我們能夠在中毒早期幫助因農藥中毒被送到急診室的患者，從他們的口腔液中進行快速床邊非侵入性之鑑定工作，提供臨床上重要的毒理學資料，以利急救過程中之關鍵性決策。

關鍵字

緊急醫療 ； 大氣質譜 ； 毒性物質辨識

並列摘要

One of the most common scenarios of acute life-threatening intoxication in the emergency department is self-poisoning with pesticides. Unfortunately, the sample pretreatment of traditional liquid chromatography-mass spectrometry technology is too time-consuming to meet the emergency medical conditions. In order to identify the toxicants involved in these aforementioned types of poison, a highly efficient analytical platform of ambient mass spectrometry have been developed with remarkable potential in solving the problem of the low efficiency of toxicant identification in emergency medicine. Self-poisoning with pesticides accounts for approximately one-third of all suicides worldwide. To expedite rescue in the emergency department, we developed an analytical method for rapid identification of ingested pesticides by thermal desorption-electrospray ionization mass spectrometry. The reproducibility of this method (n = 6) was shown in the observed low-relative standard deviation (＜ 7%) in the detection of pesticide in oral fluid. The detection limits of the pesticides in oral fluid obtained from four human subjects were between 1-10 ppb with relative standard deviation 10.7%. Linear responses of five pesticides in oral fluid with concentrations between 1 ppb-1 ppm (R^2 between 0.9938 and 0.9988) were observed. As the whole analytical process is extremely short, this technique allows for early non-invasive point-of-care identification of pesticides in the oral fluid of self-poisoning patients in the emergency room, providing important toxicological information for decision-making during critical resuscitation.

並列關鍵字

Emergency medical conditions ； Ambient mass spectrometry ； Toxicant identification