A Pilot Study: on in Vitro Maturation of CD56+ NK Cells Using the Human Umbilical Cord Blood, Among the Eleven Natural Health Substances Tested, Wheat Grass Extract Orchestrates and Enforces the Naïve Fate of CD56+ NK Cells

促進CD56+ NK細胞之天然命運及其胞內安排和促進CD56+ NK細胞的體外成熟性的中間試驗：使用人的臍帶血液和麥草萃取物測試（麥草萃取物是在被測試的十一種自然健康物質之中）

唐秉輝(Bing H. Tang) ； 謝榮峰(Jung-Feng Hsieh) ； 簡基城(Michael Chi-Chen Chien) ； 陳水田(Shui-Tein Chen)

《南臺灣醫學雜誌》 7卷2期 (2011/12) Pp. 79-93

https://doi.org/10.6726/MJST.201112_7(2).0002

摘要

目的：自然健康物質的免疫調制的活動和評估。自然健康物質它們對人的臍帶血液之中單核細胞（MNC）的作用顯示它們區別在：一般血液內大多數MNC可能更有機會被暴露在免疫致壓力素下；如果我們考慮這情況，當從人的臍帶血液（hUCB）之中的MNC有很大的不同。在人的懷孕期間，hUCB MNC與生物致壓力素由於在胎盤之間已有滋養母細胞層和它的鄰層：母親子宮內膜層已有隔絕；因此MNC對在這裡受到人的壓力已有很好的被保護了。因為滋養母細胞層防止hUBC的MNCs受各種各樣流通在母親血液因素的暴露，因此那些因素減少對這裡的MNC影響。方法：在使用十一種不同自然物質的處理以後，包括五種草本萃取物和六種純化合物，細胞的免疫表現在某一顯性特徵之生物個體或群體多（單）核細胞MNC是通過熒光啟動排序（FACS）分析的結果。本論文裡的數據報告表明了：麥草萃取物增強了CD56+自然兇手（NK）細胞的數量，而對其他MNC細胞的增強與否是無意義的。結果：NK細胞的分離法用了與正面磁性小珠細胞隔離方法，並且用麥草萃取物顯示了影響細胞改變強大，這改變明顯對人的白血病細胞系，和對K562 karyotype細胞毒性的防護增加。結論：本次中間試驗表示，麥草萃取物可能協調促進體外CD56+NK細胞（在hUCB）的天然命運及其胞內的安排，和促進體外CD56+NK細胞在hUCB的的成熟性。

關鍵字

CD56+ ； 健康物質 ； 流式細胞儀 ； K 562細胞 ； 單核細胞 ； 臍帶血液 ； 麥草萃取物 ； NK細胞

並列摘要

Objective: The immuno-modulating activity of natural substances was evaluated to assess their effects on human umbilical cord blood mononuclear cells (MNC). Most of the MNC might have been immunologically primed as they are exposed to immunological stressors, while MNC from the human umbilical cord blood (hUCB) are not. During pregnancy, MNC of hUCB is isolated from bio-stressors by virtue of the trophoblast stratum between the placenta and its neighboring maternal endometrium. Hence, the MNC is well protected from bio-stresses. The trophoblastic layer prevents MNCs of the hUBC from being affected by various factors circulating in the maternal blood, so that the exposure of MNC to those factors is reduced. Methods: After treatment with eleven different natural substances, including five herbal extracts and six pure compounds, the immunophenotypic expression of MNC subsets was analyzed via fluorescence activated cell sorting (FACS) analysis. Data reported here indicated that the extract of 79 wheat grass (Triticum aestivum L.) intensified the population of CD56^+ natural-killer (NK) cells, whereas the propagation of other MNC was insignificant. Results: NK cells separated with the positive magnetic bead cell isolation method (MACS) and treated with wheat grass extract showed marked cytotoxicity affecting the cells of Modal karyotype of human leukemia cell line, K562. Conclusions: This pilot study shows that wheat grass extracts likely orchestrates, enforces and promotes the in vitro maturation ofCD56^+ NK cells in the hUCB.

並列關鍵字

Cd56^+ ； health substances ； flow cytometry ； K 562 cells ； mononuclear cells ； umbilical cord blood ； wheat grass extract ； NK cells