新引進藥用植物之活性分析初步研究

Preliminary Analysis on Biological Activities of Newly Introduced Medicinal Plants

郭肇凱(Xhao-Kai Kuo) ； 張隆仁(Long-Zen Chang) ； 楊豐旭(Feng-Hsu Yang) ； 許輔(Fuu Sheu) ； 陳榮五(Yung-Wu Chen)

《臺中區農業改良場研究彙報》 93期 (2006/12) Pp. 1-14

https://doi.org/10.6956/BTDAIS.200612.0001

摘要

本研究配合推動藥用植物之GAP栽培技術整體計畫，針對奶薊子（Silybum marianum L., SM）、香蜂草（Melissa officinalis L., MO）、紫錐菊（Echinacea purpurea L., EP）、小白菊（Tanacetum parthenium L., TM）、紅花三葉草（Trifolium pratense L., TE）與琴葉鼠尾草 （Salvia lyrata L., SL）等六項新引進藥用植物進行抗氧化、體外免疫調節、抑制腫瘤細胞等生理活性試驗。在抗氧化分析方面，各植物水萃物之凍乾樣品在清除DPPH自由基能力、ORAC當量數、TEAC值測定等之比較結果，皆為香蜂草＞琴葉鼠尾草＞紫錐菊＞紅花三葉草＞小白菊＞奶薊子，與樣品總酚類含量有正相關，其中又以香蜂草之抗氧化力最佳。在體外免疫調節試驗方面，以紫錐菊、紅花三葉草及奶薊子三種草藥水萃物之活化巨噬細胞RAW264.7產生NO效果最佳，刺激巨噬細胞RAW264.7產生TNF-α則以香蜂草、琴葉鼠尾草及小白菊效果最為顯著。在抑制肝腫瘤細胞株之活性分析方面，對肝癌細胞Hep 3B及Hep G2之毒殺能力皆不顯著。

關鍵字

藥用植物 ； 奶薊子 ； 香蜂草 ； 紫錐菊 ； 小白菊 ； 紅花三葉草 ； 琴葉鼠尾草 ； 抗氧化 ； 免疫調節 ； 抑癌

並列摘要

The present study for meeting the core goal of the parent project The study of GAP for health care and medicinal herbs were to investigate the functionality of six medicinal plants including Silybum marianum L. (SM), Melissa officinalis L. (MO), Echinacea purpurea L. (EP), Tanacetum parthenium L. (TM), Trifolium pratense L. (TE) and Salvia lyrata L. (SL) by evaluating their antioxidative properties and capabilities to activate the immune cells of mouse and to inhibit hepatoma growth. In the evaluation of antioxidative properties, assays such as DPPH scavenging activity, TEAC and ORAC were carried out. The results of DPPH scavenging activity, TEAC and ORAC assays which were in an order of: MO＞SL＞EP＞TM＞TE＞SM, were related to their total phenolic contents. Moreover, MO showed marked antioxidant capacity and could be used for further utilization. In the immune activation capability, EP, TE and SM had the capability of activating murine macrophages RAW 264.7 to produce nitric oxide. MO, SL and TM possessed a comparable ability in increasing TNF-α production by RAW 264.7 macrophages. In evaluation of hepatoma inhibitory activity, the concentration of each sample causing 50% cytotoxicity (IC50) was determined, whereas no significant results of the cytotoxicity with Hep 3B and Hep G2 hepatoma were obtained.

並列關鍵字

medicinal plant ； Silybum marianum L. ； Melissa officinalis L. ； Echinacea purpurea L. ； Tanacetum parthenium L. ； Trifolium pratense L. ； Salvia lyrata L. ； antioxidation ； immunomodulation ； anti-tumor