- 期刊
台灣火雞鼻氣管鳥桿菌（Ornithobacterium rhinotracheale）細菌分離及血清學調查
Isolation and Serological Survey of Ornithobacterium rhinotracheale in Turkeys in Taiwan
摘要
鼻氣管鳥桿菌（Orithobacterium rhinotracheale, ORT）被認為是家禽呼吸道疾病的病原菌之一，近年來在許多國家都已分離到。本研究自2003年3月2004年12月由台灣地區台中、章化、雲林、嘉義、台南及金門等6縣15場84群不同日齡商用火雞場採取240個喉頭棉拭樣本及2160例血清樣本，上述樣本分離菌株利用16S rDNA聚合酶連鎖反應和商品化生化試驗套組API 20NE進行生化試驗與ORT標準菌株（ATCC 51464）比對鑑定，及利用商品化酵素連結免疫吸附試驗（ELISA）套組檢測火雞ORT血清抗體。結果成功分離出10株細菌（10/240）並經確證為ORT，火雞群和火雞血清盛行率3場火雞飼養場計分別為100%（16/16群）和56%（246/440隻），12場肉用火雞場計分別為69%（47/68群）和20%（338/1720隻）。本研究確認首次自台灣火雞分離出ORT菌株，並顯示台灣火雞場之高血清盛行率。
並列摘要
Ornithobacterium rhinotracheale (ORT) was considered a pathogenic bacterium of respiratory diseases in poultry and it has been isolated from many countries, but not Taiwan. A total of 240 pharyngeal swabs and of 2160 serum samples was collected from 84 flocks of varied age in 15 turkey farms (3 breeder farms, 12 meat turkey farms) in 6 counties, i.e., Taichung, Changhua, Yulln, Chiayi, Tainan and Kimen counties. The previous samples were investigated by a commercial biochemical diagnostic kit API 20NE and polymerase chain reaction (PCR) to identify the ORT and its 16S rONA, and the latter by a commercial ELISA kit to assess the epidemiological prevalence in Taiwan turkey farms. The results showed that 10 strains (10/240) were successfully isolated and finally identified as ORT. The serological prevalence of flocks and birds were 100% (16/16) and 56% (246/440) in 3 turkey breeder farms; and 69% (47/68) and 20% (338/1720) in 12 commercial meat turkey farms. Taken together, we firstly confirm the presence of ORT with a high prevalence in Taiwan turkey farms.
延伸閱讀
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