反芻動物疱疹病毒Ⅰ型及流行熱病毒混合疫苗之效力評估

Evaluate the Efficacy of Ruminant Herpesvirus Type I and Bovine Ephemeral Fever Virus Combined Inactivated Vaccines

徐淑媛(Shu-Yuen Hsu) ； 郭巧真(Chiao-Chen Kuo) ； 劉世賢(Shyh-Shyan Liu) ； 李嘉偉(Jai-Wei Lee) ； 朱純燕(Chun-Yen Chu)

《臺灣獸醫學雜誌》 37卷3期 (2011/09) Pp. 191-202

https://doi.org/10.7009/TVJ.201109.0191

摘要

Bovine herpesvirus type I (BHV-1) causes acute febrile respiratory disease in cattle, which is also known as infectious bovine rhinotracheitis (IBR). Bovine ephemeral fever virus (BEFV) also causes acute febrile respiratory disease mainly associated with pulmonary emphysema and edema. Infections caused by these two viruses in domesticated herds lead to great economic losses to the dairy farmers. In this study, BHV-1 and BEFV viruses were amplified in cultured MDBK (Maden Darby bovine kidney cells) and BHK (baby hamster kidney cell) cells, inactivated by BEI (binary ethylenimine), and formulated with water-in-oil-in-water adjuvant. No adverse reactions were observed in guinea pigs and cattle immunized with this vaccine. The titer of serum antigen-specific antibody in immunized cattle was significantly (p＜0.01) higher than that of the control group 8 weeks post primary vaccination and remained high for 12 months. In the challenge study conducted in guinea pigs, animals in the immunized group had a higher lymphocyte proliferation index than those in the control group and were protected from the challenge. Our results indicated that this vaccine formulation is effective to elicit both humoral and cell-mediated immune responses against BHV-1 and BEFV.

關鍵字

牛流行熱病毒 ； 牛疱疹病毒1型 ； 牛傳染性鼻氣管炎 ； 疫苗 ； 水包油包水佐劑

並列摘要

Bovine herpesvirus type I (BHV-1) causes acute febrile respiratory disease in cattle, which is also known as infectious bovine rhinotracheitis (IBR). Bovine ephemeral fever virus (BEFV) also causes acute febrile respiratory disease mainly associated with pulmonary emphysema and edema. Infections caused by these two viruses in domesticated herds lead to great economic losses to the dairy farmers. In this study, BHV-1 and BEFV viruses were amplified in cultured MDBK (Maden Darby bovine kidney cells) and BHK (baby hamster kidney cell) cells, inactivated by BEI (binary ethylenimine), and formulated with water-in-oil-in-water adjuvant. No adverse reactions were observed in guinea pigs and cattle immunized with this vaccine. The titer of serum antigen-specific antibody in immunized cattle was significantly (p＜0.01) higher than that of the control group 8 weeks post primary vaccination and remained high for 12 months. In the challenge study conducted in guinea pigs, animals in the immunized group had a higher lymphocyte proliferation index than those in the control group and were protected from the challenge. Our results indicated that this vaccine formulation is effective to elicit both humoral and cell-mediated immune responses against BHV-1 and BEFV.

並列關鍵字

bovine ephemeral fever ； bovine herpesvirus 1 ； infectious bovine rhinotracheitis ； vaccine ； w/o/w adjuvant